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Chèvre anti-Souris IgG (Heavy & Light Chain) Anticorps (DyLight 649) - Preadsorbed

WB, FLISA, FM Polyclonal IgG DyLight 649
N° du produit ABIN6699023
  • Antigène Tous les produits IgG
    IgG
    Épitope
    • 2332
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    • 411
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivité
    • 694
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    • 561
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    • 1
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    • 1
    Souris
    Hôte
    • 1729
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    • 15
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    • 1
    Chèvre
    Clonalité
    • 3360
    • 167
    • 1
    Polyclonal
    Conjugué
    • 557
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    DyLight 649
    Application
    • 1840
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    • 1792
    • 1500
    • 962
    • 933
    • 912
    • 515
    • 234
    • 115
    • 115
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    • 100
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    Fonction
    Goat Anti Mouse IgG (H&L) DyLight 649™ Conjugated Antibody
    Specificité
    This antibody will react with heavy chains of mouse IgG and with light chains of most mouse immunoglobulins.
    Attributs du produit

    Synonyms: Goat Anti Mouse IgG (H&L) DyLight 649™ Conjugated Antibody Pre-Adsorbed, Goat Anti-Mouse IgG Antibody DyLight 649™ Conjugation

    Background: Anti-Mouse IgG DyLight 649 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

    Purification
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. 
    Immunogène
    Mouse IgG whole molecule
    Isotype
    IgG
    Labeling Ratio
    2.7
  • Indications d'application

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    0.01 % (w/v) Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Date de péremption
    12 months
  • Manning, Bundros, Trimmer: "Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importance." dans: PLoS ONE, Vol. 7, Issue 6, pp. e38313, (2012) (PubMed).

  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
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