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Lapin anti-Souris IgG2a (Chain gamma 2a) Anticorps (DyLight 800) - Preadsorbed

WB, FLISA, FM Polyclonal IgG DyLight 800
N° du produit ABIN6699066
  • Antigène Tous les produits IgG2a
    IgG2a
    Épitope
    • 43
    • 14
    • 7
    • 2
    • 2
    • 1
    • 1
    Chain gamma 2a
    Reactivité
    • 93
    • 18
    • 1
    Souris
    Hôte
    • 57
    • 22
    • 15
    • 13
    • 5
    Lapin
    Clonalité
    • 71
    • 28
    Polyclonal
    Conjugué
    • 20
    • 12
    • 11
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    DyLight 800
    Application
    • 50
    • 47
    • 34
    • 30
    • 23
    • 18
    • 10
    • 8
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    Attributs du produit

    Synonyms: Rabbit Anti-Mouse IgG2a (Gamma 2a chain) Antibody DyLight 800™ Conjugated, Rabbit Anti Mouse IgG2a DyLight 800™ Conjugated Antibody

    Background: Anti-Mouse IgG2a DyLight 800 Antibody generated in rabbit detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Mouse IgG2a heavy chain

    Isotype
    IgG
    Labeling Ratio
    2.0
  • Indications d'application

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    0.01 % (w/v) Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Date de péremption
    12 months
  • Antigène
    IgG2a
    Abstract
    IgG2a Produits
    Classe de substances
    Antibody
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