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Lapin anti-Souris IgG3 (Chain gamma 3) Anticorps (Cy3) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG Cy3
N° du produit ABIN6699076
  • Antigène Tous les produits IgG3
    IgG3
    Épitope
    • 38
    • 14
    • 6
    • 5
    • 4
    • 1
    • 1
    • 1
    Chain gamma 3
    Reactivité
    • 70
    • 19
    • 3
    Souris
    Hôte
    • 37
    • 27
    • 17
    • 10
    • 1
    Lapin
    Clonalité
    • 63
    • 27
    Polyclonal
    Conjugué
    • 18
    • 12
    • 11
    • 10
    • 6
    • 5
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cy3
    Application
    • 51
    • 40
    • 36
    • 31
    • 26
    • 20
    • 12
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Attributs du produit

    Synonyms: Rabbit Anti-Mouse IgG3 (Gamma 3 chain) CY3 Conjugated Antibody, Rabbit Anti Mouse IgG3 Antibody CY3 Conjugation

    Background: Anti-Mouse IgG3 Cy3 Antibody generated in rabbit detects reactivity to Mouse IgG3 (Gamma 3 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Mouse IgG3 heavy chain

    Isotype
    IgG
    Labeling Ratio
    8.36
  • Indications d'application

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    0.01 % (w/v) Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Date de péremption
    12 months
  • Antigène
    IgG3
    Abstract
    IgG3 Produits
    Classe de substances
    Antibody
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