Chèvre anti-Souris IgM (Chain mu) Anticorps (DyLight 680) - Preadsorbed

N° du produit ABIN6699087

Détail du produit

Antigène: IgM

Épitope: Chain mu

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: DyLight 680

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Fonction: Goat Anti-Mouse IgM (mu chain) Antibody DyLight 680™ Conjugated

Réactivité croisée (Details): No reaction was observed against other mouse heavy or light chain proteins.

Attributs du produit:

Synonyms: Goat Anti-Mouse IgM (mu chain) Antibody DyLight 680™ Conjugated, Goat Anti Mouse IgM mu chain DyLight 680™ Conjugated Antibody

Background: Anti-Mouse IgM DyLight antibody specifically detects mouse IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. 

Immunogène: Mouse IgM whole molecule

Isotype: IgG

Labeling Ratio: 2.1

Information d'application

Indications d'application:

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgM

Abstract: IgM Produits

Classe de substances: Antibody