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Chèvre anti-Lapin IgG Anticorps (Cy5) - Preadsorbed

WB, FACS, FLISA, FM Polyclonal IgG Cy5
N° du produit ABIN6699092
  • Antigène Tous les produits IgG
    IgG
    Reactivité
    • 695
    • 672
    • 560
    • 458
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 97
    • 95
    • 92
    • 62
    • 47
    • 38
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Lapin
    Hôte
    • 1729
    • 1474
    • 539
    • 206
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Chèvre
    Clonalité
    • 3360
    • 167
    • 1
    Polyclonal
    Conjugué
    • 557
    • 409
    • 406
    • 368
    • 298
    • 276
    • 228
    • 121
    • 99
    • 67
    • 66
    • 62
    • 62
    • 52
    • 48
    • 47
    • 45
    • 44
    • 44
    • 40
    • 39
    • 34
    • 33
    • 33
    • 31
    • 31
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    • 29
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    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
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    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    Cy5
    Application
    • 1840
    • 1799
    • 1792
    • 1500
    • 962
    • 933
    • 912
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 52
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Attributs du produit

    Synonyms: Goat Anti Rabbit IgG Antibody CY5 Conjugation, Goat Anti-Rabbit IgG CY5 Conjugated Antibody

    Background: Anti-Rabbit IgG Antibody CY5 generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to CY5.

    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Rabbit IgG whole molecule

    Isotype
    IgG
    Labeling Ratio
    17.2
  • Indications d'application

    Application Note: This product is designed for flow cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    Western Blot Dilution: User Optimized

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    0.01 % (w/v) Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Date de péremption
    12 months
  • Richardson, Winistorfer, Poupon, Luzio, Piper: "Mammalian late vacuole protein sorting orthologues participate in early endosomal fusion and interact with the cytoskeleton." dans: Molecular biology of the cell, Vol. 15, Issue 3, pp. 1197-210, (2004) (PubMed).

  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
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