BAD anticorps (BCL2-Associated Agonist of Cell Death) (AA 100-145)

Details for Product anti-BAD Antibody No. ABIN6712220
Antigène
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
  • BCL2-associated agonist of cell death b
  • BCL2 associated agonist of cell death
  • BCL2-associated agonist of cell death
  • badb
  • BAD
  • Bad
Épitope
AA 100-145
66
58
55
50
46
46
45
40
27
25
24
24
17
17
17
17
17
17
17
16
16
16
16
16
16
16
16
16
16
16
15
14
14
11
9
9
9
9
9
9
9
6
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
806
491
341
20
19
8
5
4
3
2
2
1
1
1
1
1
Hôte
Lapin
990
43
2
1
Clonalité
Polyclonal
Conjugué
Cet anticorp BAD est non-conjugé
74
67
67
63
62
61
12
12
12
12
12
12
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC)
552
530
366
361
98
79
42
32
14
13
10
8
6
3
2
1
1
1
1
Options
Immunogène Synthetic peptide, corresponding to amino acids 100-145 of Human Bad.
Specificité BAD (P130) polyclonal antibody detects endogenous levels of BAD protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pureté > 95% (by SDS-PAGE)
Plasmids, Primers & others Plasmids, Primers & others BAD products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Bad (BAD Antibody Extrait)
Sujet Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Poids moléculaire ~ 24 kDa
UniProt Q92934
Pathways Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, Apoptose, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events
Indications d'application IHC: 1:50~1:200 IF: 1:50~1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 1 mg/mL in Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles.
Stock 4 °C,-20 °C
Stockage commentaire Store at 4°C short term. Aliquot and store at -20°C long term.
Produit citée dans: Li, Ying, Wang, Li, Fu, Guo, Xu: "Microcystin-LR induces ceramide to regulate PP2A and destabilize cytoskeleton in HEK293 cells." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 128, Issue 1, pp. 147-57, 2012 (PubMed).

Yang, Liu, Fu: "Effects of PEG-liposomal oxaliplatin on apoptosis, and expression of Cyclin A and Cyclin D1 in colorectal cancer cells." dans: Oncology reports, Vol. 28, Issue 3, pp. 1006-12, 2012 (PubMed).

Li, Huang, Liang, Fu, Guo, Xu: "Microcystin-LR (MCLR) induces a compensation of PP2A activity mediated by ?4 protein in HEK293 cells." dans: International journal of biological sciences, Vol. 7, Issue 6, pp. 740-52, 2011 (PubMed).

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