BAD anticorps (BCL2-Associated Agonist of Cell Death)

Details for Product anti-BAD Antibody No. ABIN6714980
Antigène
Reactivité
Humain, Souris, Rat (Rattus)
795
473
322
20
17
8
5
4
3
2
2
1
1
1
1
1
Hôte
Lapin
986
40
2
1
Clonalité
Polyclonal
Conjugué
Cet anticorp BAD est non-conjugé
74
66
66
63
62
61
12
12
12
12
12
12
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
545
521
381
333
110
79
35
34
19
19
8
7
5
3
2
1
1
1
1
Options
Immunogène Recombinant full length Human BAD.
Specificité BAD polyclonal antibody detects endogenous levels of BAD protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pureté > 95% (by SDS-PAGE)
Antigène
Autre désignation BAD (BAD Antibody Extrait)
Sujet Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL. Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL. Akt phosphorylates Bad at Ser136 to promote cell survival. Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK and mitochondria-anchored PKA. Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL.
Poids moléculaire ~ 18, 24 kDa
UniProt Q92934
Pathways Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, Apoptose, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events
Indications d'application WB: 1:500~1:2000 IHC/IF: 1:50~1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 1 mg/mL in Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles.
Stock 4 °C,-20 °C
Stockage commentaire Store at 4°C short term. Aliquot and store at -20°C long term.
Images (Fournisseur)
Image no. 1 for anti-BCL2-Associated Agonist of Cell Death (BAD) antibody (ABIN6714980) Western blot (WB) analysis of BAD pAb
Produit citée dans: Li, Ying, Wang, Li, Fu, Guo, Xu: "Microcystin-LR induces ceramide to regulate PP2A and destabilize cytoskeleton in HEK293 cells." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 128, Issue 1, pp. 147-57, 2012 (PubMed).

Yang, Liu, Fu: "Effects of PEG-liposomal oxaliplatin on apoptosis, and expression of Cyclin A and Cyclin D1 in colorectal cancer cells." dans: Oncology reports, Vol. 28, Issue 3, pp. 1006-12, 2012 (PubMed).

Li, Huang, Liang, Fu, Guo, Xu: "Microcystin-LR (MCLR) induces a compensation of PP2A activity mediated by ?4 protein in HEK293 cells." dans: International journal of biological sciences, Vol. 7, Issue 6, pp. 740-52, 2011 (PubMed).