NFIC anticorps (AA 323-372)

N° du produit ABIN6744464

Détail du produit anti-NFIC anticorps

Antigène: NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))

Épitope: AA 323-372

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Cobaye, Porc, Singe, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NFIC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human NFIC

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Chicken (93%) Xenopus (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa323-372 of human NFIC (Q6FI30, NP_995315). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Pig, Guinea pig (100%), Zebra finch, Chicken (93%), Opossum (92%), Xenopus (86%), Stickleback (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Aliquot to Avoid freeze/thaw cycles after reconstitution

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur NFIC

Antigène: NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))

Autre désignation: NFI / NFIC (NFIC Produits)

Synonymes: anticorps LOC100005568, anticorps 1110019L22Rik, anticorps 1500041O16Rik, anticorps AA589446, anticorps AI746521, anticorps NF1-C, anticorps CTF, anticorps CTF5, anticorps NF-I, anticorps NFI, anticorps CNFI-C, anticorps nf1-c1, anticorps nuclear factor I/C, anticorps nuclear factor I C, anticorps nuclear factor I C L homeolog, anticorps nfic, anticorps NFIC, anticorps Nfic, anticorps nfic.L

Sujet: Name/Gene ID: NFIC

Synonyms: NFIC, CTF, CTF5, NF1-C, NF-I/C, NFI, NFI-C, TGGCA-binding protein, NF-I, Nuclear factor 1 C-type, Nuclear factor 1/C, Nuclear factor I/C

ID gène: 4782

NCBI Accession: NP_995315

UniProt: P08651