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TJP1 anticorps (AA 1551-1702)

TJP1 Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache) WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN675024
  • Antigène Voir toutes TJP1 Anticorps
    TJP1 (Tight Junction Protein 1 (TJP1))
    Épitope
    • 15
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1551-1702
    Reactivité
    • 54
    • 31
    • 29
    • 19
    • 17
    • 9
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Porc, Boeuf (Vache)
    Hôte
    • 48
    • 4
    • 3
    • 1
    • 1
    Lapin
    Clonalité
    • 50
    • 7
    Polyclonal
    Conjugué
    • 39
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp TJP1 est non-conjugé
    Application
    • 52
    • 19
    • 18
    • 14
    • 13
    • 13
    • 12
    • 11
    • 11
    • 7
    • 7
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))
     Réactivité croisée
    Boeuf (Vache), Humain, Souris, Porc, Rat
    Homologie
    Dog,Cow,Chicken,Rabbit,Guinea Pig
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human ZO-1
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029577 (Immunohistochemistry)
    'Independent Validation' signe
    by
    Immunohistochemistry Core, NYU Langone
    No.
    #029577
    Date
    18.01.2014
    Antigène
    Numéro du lot
    130913
    Application validée
    Immunohistochemistry
    Contrôle positif
    Testis
    Contrôle négative
    See Controls section for negative controls
    Conclusion
    Signal was detected in positive control tissue, and not detected in a tissue expected to express very low levels of the target antigen.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antibody: human Tight Junction Protein 1 (Zona Occludens 1) (TJP1)
    • Catalog number: ABIN675024
    • Lot number: 130913
    Anticorps secondaire
    • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
    • Lot number: D05923BA
    Full Protocol
    • Immunohistochemistry was performed on a Ventana NexES automated platform, instrument manufacturer specific reagents are italicized.
    • 1. Slides were preheated in convection oven at 60°C for 30 minutes
    • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 minutes each - 3 changes of 100% Ethanol, 3 minutes each - 3 changes of 95% Ethanol, 3 minutes each - Rinsed in distilled water, 3 changes
    • 1. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 minutes. - Slides were allowed to cool (in citrate) for 30 minutes. - Slides were washed x 3 in Distilled water
    • 1. NexES instrument procedure, iVIEW DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
    • 1. Slides rinsed with *reaction buffer* x 3
    • 1. *iVIEW Inhibitor (H2O2)* applied and incubated for 4 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliters applied/slide) - Ventana Isotype control applied neat - Slides incubated overnight at room temperature (~12 hours ~25°C)
    • 1. Slides rinsed with *reaction buffer* x3
    • 1. *iVIEW Biotinylated IgG* applied and incubated for 8 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. *iVIEW Streptavidin-Horseradish Peroxidase* applied and incubated for 8 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. *iVIEW DAB/H2O2* applied and incubated for 8 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. *iVIEW Copper* applied and incubated for 4 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. Slides washed in Dawn Detergent/tap water
    • 1. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 seconds - Slides washed in tap water, 1 minute - Decolorized (10% Acetic Acid in 70% ethanol), 1 minute - Slides washed in tap water, 1 minute - Bluing (Austin Clear Ammonia), 1 minute - Slides washed in tap water, 1 minute
    • 1. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 minutes each - 3 changes of 100% Ethanol, 3 minutes each - 3 changes of Xylene, 5 minutes each - Mounted with Permount
    • 1. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
    Notes
    • Deviations from protocol/procedure supplied by manufacturer (attached).
    • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
    • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
    • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
    • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
    • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
    • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
    • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
    • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
    • Tissue Interpretation (limited): - TJP1: Under the staining parameters described above, testis stained weakly (ducts) positive (Figure 1). Substantial signal detected in limited number of other tissues, including: breast, normal (NOS); pancreatic cancer (NOS), and stomach, normal (NOS). Most tissues showed low level of specific signal. Thymus did not have any detectable signal (Figure 4).
    • I-NC (Isotype negative control): No signal detected
    • B-NC (Blank negative control): No signal detected
    • Signal Localization: - Signal to noise was adequate with cytoplasmic, nuclear subcellular localization observed. Rare inner-membrane and no distinct membrane signal observed.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Zhuang, Zhong, Bian, Fan, Chen, Liu, Liu: "Rhein ameliorates lipopolysaccharide-induced intestinal barrier injury via modulation of Nrf2 and MAPKs." dans: Life sciences, Vol. 216, pp. 168-175, (2019) (PubMed).

    Kulhankova, Kinney, Stach, Gourronc, Grumbach, Klingelhutz, Salgado-Pabón: "The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function." dans: Infection and immunity, (2018) (PubMed).

    Zou, Lin, Li, Wu, He, Huang, Wang, Ye, Cheng, Ding, Zheng, Chi: "Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response." dans: Scientific reports, Vol. 6, pp. 39299, (2018) (PubMed).

    Zhao, Qin, Han, Wang, Zhang, Liu: "?-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2." dans: International journal of molecular sciences, Vol. 15, Issue 2, pp. 1915-26, (2014) (PubMed).

    Ruan, Liu, Zhou, Mi, Liu, Wu, Yao, Assaad, Deng, Hou, Wu, Yin: "Chlorogenic acid decreases intestinal permeability and increases expression of intestinal tight junction proteins in weaned rats challenged with LPS." dans: PLoS ONE, Vol. 9, Issue 6, pp. e97815, (2014) (PubMed).

    Zhao, Qin, Sun, Che, Bao, Zhang: "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets." dans: International journal of molecular sciences, Vol. 12, Issue 12, pp. 8502-12, (2012) (PubMed).

    Gu, Xue, Wei, Zhang, Li: "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." dans: Neuroscience letters, Vol. 493, Issue 3, pp. 140-4, (2011) (PubMed).

  • Antigène
    TJP1 (Tight Junction Protein 1 (TJP1))
    Autre désignation
    ZO-1 (TJP1 Produits)
    Synonymes
    anticorps ZO-1, anticorps ZO1, anticorps zo1, anticorps tight junction protein 1, anticorps tight junction protein ZO-1, anticorps TJP1, anticorps Tjp1, anticorps LOC396567
    Sujet

    Synonyms: ZO-1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1, ZO1

    Background: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

    ID gène
    7082
    UniProt
    Q07157
    Pathways
    Carbohydrate Homeostasis, Cell-Cell Junction Organization
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