MTOR anticorps (AA 2436-2492)

N° du produit ABIN676403

Détail du produit anti-MTOR anticorps

Antigène: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Épitope: AA 2436-2492

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MTOR est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Poulet, Boeuf (Vache), Poisson, Humain, Souris, Rat

Homologie: Dog,Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human mTOR

Isotype: IgG

Information d'application

Indications d'application: IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Independent Validation (WB)

Validation #029761 (Western Blotting)

by: Alamo Laboratories Inc

No.: #029761

Date: 03.07.2014

Numéro du lot: 131209

Application validée: Western Blotting

Contrôle positif: HeLa cells

Contrôle négative: c6/36 mosquito cells (non-reactive species)

Conclusion: A strong band was observed in the positive control sample at the correct molecular weight. Multiple faint bands were also observed in the positive sample, but these bands did not represent a significant background signal. A band at the correct molecular weight was also observed in the negative control; however, it may be impossible to find a true negative control for this antigen.

Validation Images

Figure 1. Western blot of lysates from HeLa cells (Lane 1) and c6/36 cells (Lane 2) probed with anti-mTOR (upper panel) or with anti-Actin for loading control (lower panel).

Protocole

Anticorps primaire:

• Antigen: Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (1:200 dilution)
• Catalog number: ABIN676403
• Lot number: 131209

Anticorps secondaire:

• Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
• Lot number: L170-6515

Full Protocol:

• Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
• 20 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
• The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
• Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
• The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 h.
• The membrane was rinsed with TBST once.
• The membrane was immersed with the protein side up in the primary antibody solution (FRAP1; 1:200) in TBST containing 5% (W/V) BSA and incubated for 16 h at 4°C.
• The membrane was rinsed in TBST thrice for 5 min each.
• The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) BSA and incubated for 1 h at room temperature (~26°C) with gentle agitation.
• The membrane was rinsed thrice TBST thrice for 5 min each.
• The membrane was rinsed in TBS twice for 30 s each.
• Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
• The membrane was rinsed three times TBST.
• Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
• The membrane was washed in TBST 2 times for 10 min each.
• Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).

Notes: - No experimental challenges noted.

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-MTOR (ABIN676403)

Wang, Sun, Han, Li, Ge, Zhao, De Felici, Shen, Cheng: "Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis." dans: Aging, Vol. 10, Issue 3, pp. 463-480, (2019) (PubMed).

Sun, Li, Zhao, Wang, Liu, Shao, Xue, Xing: "Synergistic effect of copper and arsenic upon oxidative stress, inflammation and autophagy alterations in brain tissues of Gallus gallus." dans: Journal of inorganic biochemistry, Vol. 178, pp. 54-62, (2018) (PubMed).

Li, Qiu, Liu, Wang, Hu, Gan, Wang: "Long-term thermal manipulation in the late incubation period can inhibit breast muscle development by activating endoplasmic reticulum stress in duck (Anasplatyrhynchos domestica)." dans: Journal of thermal biology, Vol. 70, Issue Pt B, pp. 37-45, (2017) (PubMed).

Tian, Liu, Qu: "Role of endoplasmic reticulum stress on cisplatin resistance in ovarian carcinoma." dans: Oncology letters, Vol. 13, Issue 3, pp. 1437-1443, (2017) (PubMed).

Zou, Geng, Chen, Ren, Liu, Wan, Guo, Wang: "Ranolazine improves oxidative stress and mitochondrial function in the atrium of acetylcholine-CaCl2 induced atrial fibrillation rats." dans: Life sciences, Vol. 156, pp. 7-14, (2016) (PubMed).

Madka, Mohammed, Li, Zhang, Biddick, Patlolla, Lightfoot, Towner, Wu, Steele, Kopelovich, Rao: "Targeting mTOR and p53 Signaling Inhibits Muscle Invasive Bladder Cancer In Vivo." dans: Cancer prevention research (Philadelphia, Pa.), Vol. 9, Issue 1, pp. 53-62, (2016) (PubMed).

Yang, Wang, Wang, Zhang, Zhang, Lu, Wang: "mTOR is involved in 17?-estradiol-induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." dans: Molecular reproduction and development, Vol. 82, Issue 4, pp. 305-14, (2015) (PubMed).

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." dans: Bioscience reports, Vol. 34, Issue 5, (2014) (PubMed).

Détails sur MTOR

Antigène: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Autre désignation: Mtor/Frap (mTOR Produits)

Synonymes: anticorps FRAP1, anticorps FRAP, anticorps FRAP2, anticorps RAFT1, anticorps RAPT1, anticorps 2610315D21Rik, anticorps AI327068, anticorps Frap1, anticorps flat, anticorps frap1, anticorps tor, anticorps wu:fc22h08, anticorps mechanistic target of rapamycin kinase, anticorps mechanistic target of rapamycin, anticorps mechanistic target of rapamycin (serine/threonine kinase), anticorps MTOR, anticorps Mtor, anticorps mtor

Sujet:

Synonyms: Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Background: The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

ID gène: 2475

UniProt: P42345

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, L'effet Warburg