Tumor Necrosis Factor anticorps (TNF) (AA 180-230)

Details for Product anti-TNF Antibody No. ABIN677318
Antigène
Épitope
AA 180-230
82
47
46
44
23
15
14
8
8
7
7
7
7
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
936
364
285
117
112
96
95
56
51
40
26
17
15
6
6
6
5
4
4
3
3
3
2
2
1
1
1
1
Hôte
Lapin
675
488
78
48
10
6
6
5
2
2
2
2
Clonalité
Polyclonal
Conjugué
Cet anticorp Tumor Necrosis Factor est non-conjugé
160
71
35
27
20
11
11
11
11
11
11
11
11
11
11
11
11
11
9
8
7
7
7
7
7
3
2
2
2
2
2
2
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
654
618
419
390
244
125
118
87
71
69
63
59
32
26
25
19
18
16
14
9
8
8
7
6
6
6
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
Options
'Independent Validation' signe
Numéro du lot 140113
Application validée Immunofluorescence
Contrôle positif Normal mouse lung tissue
Contrôle négative Normal mouse adipose tissue
Conclusion Fluorescent signal is detected in positive control, but not in negative control or isotype controls.
Anticorps primaire
  • Antibody: Tumor Necrosis Factor (TNF)
  • Catalog number: ABIN677318
  • Lot number: 140113
Contrôle isotypique
  • Antibody: Rabbit IgG Isotype
  • Lot number: not available
Anticorps secondaire
  • Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)
  • Lot number: Not available
Contrôles
  • Positive control: FFPE normal mouse lung tissue
  • Negative Control: FFPE normal mouse fat tissue
  • Isotype antibody control: tissue sections treated with Rabbit IgG Isotype at 1ug/ul
  • Secondary antibody only control: positive and negative control tissue sections treated with Goat anti-Rabbit Alexa 647 secondary antibody only. Any staining observed is due to non-specific binding of secondary antibody.
Protocole
  • Paraffin embedded positive and negative control tissue sections were deparaffinized and underwent antigen retrieval using 10 mM sodium citrate pH 6.0 in a pressure cooker for 10 min.
  • The tissue sections were incubated with 1 mg/mL sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
  • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
  • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen).
  • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
Notes Nothing to note.
Validation Images
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 1: confocal image of positive control (mouse lung) with anti-TNF alpha antibody
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 2: confocal image of negative control (mouse adipose) with anti-TNF alpha antib...
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 3: confocal image of positive control (mouse lung) with isotype control
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 4: confocal image of negative control (mouse adipose) with isotype control
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 5: confocal image of positive control (mouse lung) with secondary antibody only
Immunogène KLH conjugated synthetic peptide derived from mouse TNF-alpha
Isotype IgG
Purification Purified by Protein A.
Antigène
Autre désignation Tnf alpha (TNF Antibody Extrait)
Sujet

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Subcellular location: Extracellular

Synonyms: DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tnf

ID gène 21926
UniProt P06804
Pathways Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Indications d'application WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20°C
Date de péremption 12 months
Images (Fournisseur)
Image no. 1 for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha P...
Image no. 2 for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alp...
Image no. 3 for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha P...
Image no. 4 for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300...
Produit citée dans: Lennikov, Mirabelli, Mukwaya, Schaupper, Thangavelu, Lachota, Ali, Jensen, Lagali: "Selective IKK2 inhibitor IMD0354 disrupts NF-κB signaling to suppress corneal inflammation and angiogenesis." dans: Angiogenesis, Vol. 21, Issue 2, pp. 267-285, 2019 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Wang, Tang, Shen, Chen, Chen, Luo, Ge: "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." dans: Cytokine, Vol. 99, pp. 275-280, 2018 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Mouse (Murine)).

Li, Fang, Tian, Wang, Ao, Hou, Tong, Fan, Bai: "Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation." dans: Molecular medicine reports, Vol. 16, Issue 4, pp. 4455-4462, 2018 (PubMed). Méthode utilisée: Western Blotting (WB)

Dong, Zhou, Ji, Pan, Zheng: "AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE-/- mice." dans: Biochemical and biophysical research communications, Vol. 489, Issue 4, pp. 426-431, 2017 (PubMed). Méthode utilisée: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Échantillon (espèces): Mouse (Murine)).

Han, Wang, Qin, Xia, Wu: "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." dans: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 83, 2017 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Wang, Li, Liu, Wang, Cao: "Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin‑induced lung inflammation in a murine model of asthma." dans: Molecular medicine reports, Vol. 13, Issue 5, pp. 4289-302, 2017 (PubMed). Méthode utilisée: Western Blotting (WB), Western Blotting (WB)

Shi, Yu, Liu, Liu, Zhang, Yang, Chai, Lou, Gao, Zhu: "Panax notoginseng saponins administration modulates pro- /anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats." dans: Metabolic brain disease, Vol. 32, Issue 1, pp. 221-233, 2016 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Rat (Rattus)). Détails: Sample: brain

Ji, Wang, Wang, Chen, Li: "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits." dans: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 51-58, 2016 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Rabbit). Détails: Sample: lung

Du, Niu, Wang, Zhao, Kong, Zhang, Nan: "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." dans: Molecular medicine reports, Vol. 13, Issue 3, pp. 2159-66, 2016 (PubMed). Méthode utilisée: Western Blotting (WB), Western Blotting (WB) (Échantillon (espèces): Mouse (Murine)). Détails: Sample: liver

Bertuglia, Pagliara, Grego, Ricci, Brkljaca-Bottegaro: "Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career." dans: BMC veterinary research, Vol. 12, Issue 1, pp. 246, 2016 (PubMed). Méthode utilisée: ELISA (ELISA), ELISA (ELISA)

Wang, Li, Wei, Xu, Chen, Song, Lu, Wang: "Targeted knockout of TNF?? by injection of lentivirus?mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats." dans: Molecular medicine reports, Vol. 12, Issue 3, pp. 4389-95, 2015 (PubMed).

Sun, Zeng, Cao, Song, Zhang, Liu: "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." dans: BioMed research international, Vol. 2015, pp. 919401, 2015 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Rat (Rattus)).

Xu, Zhao, Lin, Wang, Hu, Jiang: "Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus." dans: BMC ophthalmology, Vol. 15, pp. 126, 2015 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Rat (Rattus)).

Fang, Li, Sun, Bao, Ren, Lv, Ma: "Ischemic preconditioning protects against spinal cord ischemia-reperfusion injury in rabbits by attenuating blood spinal cord barrier disruption." dans: International journal of molecular sciences, Vol. 14, Issue 5, pp. 10343-54, 2015 (PubMed). Méthode utilisée: Western Blotting (WB), Western Blotting (WB)

Luo, Wang, Li, Wei, Zhang, Liu, Wang, Niu, Wang: "Maternal bisphenol a diet induces anxiety-like behavior in female juvenile with neuroimmune activation." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 140, Issue 2, pp. 364-73, 2015 (PubMed). Méthode utilisée: Western Blotting (WB), Western Blotting (WB)

Morishita, Oku, Horie, Tonari, Kida, Okubo, Sugiyama, Takai, Hara, Ikeda: "Systemic simvastatin rescues retinal ganglion cells from optic nerve injury possibly through suppression of astroglial NF-?B activation." dans: PLoS ONE, Vol. 9, Issue 1, pp. e84387, 2014 (PubMed). Méthode utilisée: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Échantillon (espèces): Rat (Rattus)).

Wang, Hu, Wang, Gu, Gao, Cao, Zheng: "5-Aminolevulinic acid-mediated sonodynamic therapy reverses macrophage and dendritic cell passivity in murine melanoma xenografts." dans: Ultrasound in medicine & biology, Vol. 40, Issue 9, pp. 2125-33, 2014 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Mouse (Murine)).

Wang, Zhang, Gu, Ding, Fan, Zhu, Shi, Hong, Kong: "Morin reduces hepatic inflammation-associated lipid accumulation in high fructose-fed rats via inhibiting sphingosine kinase 1/sphingosine 1-phosphate signaling pathway." dans: Biochemical pharmacology, Vol. 86, Issue 12, pp. 1791-804, 2013 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Rat (Rattus)). Détails: Sample: liver