TNF alpha anticorps (AA 181-235)

N° du produit ABIN677318

Détail du produit anti-TNF alpha anticorps

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Épitope: AA 181-235

Reactivité: Humain, Souris, Rat, Lapin, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Cheval, Humain, Souris, Lapin, Rat

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from mouse TNF-alpha

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200

Restrictions: For Research Use only

Independent Validation (IF)

Validation #029732 (Immunofluorescence)

by: Confocal Imaging Core, Beth Israel Deaconess Medical Center

No.: #029732

Date: 12.06.2014

Numéro du lot: 140113

Application validée: Immunofluorescence

Contrôle positif: Normal mouse lung tissue

Contrôle négative: Normal mouse adipose tissue

Conclusion: Fluorescent signal is detected in positive control, but not in negative control or isotype controls.

Validation Images

Panel 1: confocal image of positive control (mouse lung) with anti-TNF alpha antibody

Panel 2: confocal image of negative control (mouse adipose) with anti-TNF alpha antibody

Panel 3: confocal image of positive control (mouse lung) with isotype control

Panel 4: confocal image of negative control (mouse adipose) with isotype control

Panel 5: confocal image of positive control (mouse lung) with secondary antibody only

Protocole

Anticorps primaire:

• Antibody: Tumor Necrosis Factor (TNF)
• Catalog number: ABIN677318
• Lot number: 140113

Anticorps secondaire:

• Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)
• Lot number: Not available

Full Protocol:

• Paraffin embedded positive and negative control tissue sections were deparaffinized and underwent antigen retrieval using 10 mM sodium citrate pH 6.0 in a pressure cooker for 10 min.
• The tissue sections were incubated with 1 mg/mL sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
• Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
• Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
• Tissue sections were rinsed three times in TBS for 5 min each at RT.
• Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
• Tissue sections were rinsed three times in TBS for 5 min each at RT.
• Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen).
• IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.

Notes: Nothing to note.

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-TNF alpha (ABIN677318)

Maier, Reichhart, Gonnermann, Kociok, Riechardt, Gundlach, Strauß, Joussen: "Effects of TNFα receptor TNF-Rp55- or TNF-Rp75- deficiency on corneal neovascularization and lymphangiogenesis in the mouse." dans: PLoS ONE, Vol. 16, Issue 4, pp. e0245143, (2021) (PubMed).

Mousa, Soliman, Alhumaydhi, Almatroudi, Al Rugaie, Allemailem, Alrumaihi, Khan, Rezk, Aljasir, Alwashmi, Aba Alkhayl, Albutti, Seleem: "Garlic Extract Alleviates Trastuzumab-Induced Hepatotoxicity in Rats Through Its Antioxidant, Anti-Inflammatory, and Antihyperlipidemic Effects." dans: Journal of inflammation research, Vol. 14, pp. 6305-6316, (2021) (PubMed).

Lennikov, Mirabelli, Mukwaya, Schaupper, Thangavelu, Lachota, Ali, Jensen, Lagali: "Selective IKK2 inhibitor IMD0354 disrupts NF-κB signaling to suppress corneal inflammation and angiogenesis." dans: Angiogenesis, Vol. 21, Issue 2, pp. 267-285, (2019) (PubMed).

Han, Wang, Qin, Xia, Wu: "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." dans: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 83, (2018) (PubMed).

Wang, Tang, Shen, Chen, Chen, Luo, Ge: "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." dans: Cytokine, Vol. 99, pp. 275-280, (2018) (PubMed).

Li, Fang, Tian, Wang, Ao, Hou, Tong, Fan, Bai: "Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation." dans: Molecular medicine reports, Vol. 16, Issue 4, pp. 4455-4462, (2018) (PubMed).

Dong, Zhou, Ji, Pan, Zheng: "AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE-/- mice." dans: Biochemical and biophysical research communications, Vol. 489, Issue 4, pp. 426-431, (2017) (PubMed).

Wang, Li, Liu, Wang, Cao: "Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin‑induced lung inflammation in a murine model of asthma." dans: Molecular medicine reports, Vol. 13, Issue 5, pp. 4289-302, (2017) (PubMed).

Shi, Yu, Liu, Liu, Zhang, Yang, Chai, Lou, Gao, Zhu: "Panax notoginseng saponins administration modulates pro- /anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats." dans: Metabolic brain disease, Vol. 32, Issue 1, pp. 221-233, (2016) (PubMed).

Ji, Wang, Wang, Chen, Li: "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits." dans: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 51-58, (2016) (PubMed).

Du, Niu, Wang, Zhao, Kong, Zhang, Nan: "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." dans: Molecular medicine reports, Vol. 13, Issue 3, pp. 2159-66, (2016) (PubMed).

Bertuglia, Pagliara, Grego, Ricci, Brkljaca-Bottegaro: "Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career." dans: BMC veterinary research, Vol. 12, Issue 1, pp. 246, (2016) (PubMed).

Wang, Li, Wei, Xu, Chen, Song, Lu, Wang: "Targeted knockout of TNF?? by injection of lentivirus?mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats." dans: Molecular medicine reports, Vol. 12, Issue 3, pp. 4389-95, (2015) (PubMed).

Sun, Zeng, Cao, Song, Zhang, Liu: "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." dans: BioMed research international, Vol. 2015, pp. 919401, (2015) (PubMed).

Xu, Zhao, Lin, Wang, Hu, Jiang: "Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus." dans: BMC ophthalmology, Vol. 15, pp. 126, (2015) (PubMed).

Fang, Li, Sun, Bao, Ren, Lv, Ma: "Ischemic preconditioning protects against spinal cord ischemia-reperfusion injury in rabbits by attenuating blood spinal cord barrier disruption." dans: International journal of molecular sciences, Vol. 14, Issue 5, pp. 10343-54, (2015) (PubMed).

Luo, Wang, Li, Wei, Zhang, Liu, Wang, Niu, Wang: "Maternal bisphenol a diet induces anxiety-like behavior in female juvenile with neuroimmune activation." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 140, Issue 2, pp. 364-73, (2015) (PubMed).

Morishita, Oku, Horie, Tonari, Kida, Okubo, Sugiyama, Takai, Hara, Ikeda: "Systemic simvastatin rescues retinal ganglion cells from optic nerve injury possibly through suppression of astroglial NF-?B activation." dans: PLoS ONE, Vol. 9, Issue 1, pp. e84387, (2014) (PubMed).

Wang, Hu, Wang, Gu, Gao, Cao, Zheng: "5-Aminolevulinic acid-mediated sonodynamic therapy reverses macrophage and dendritic cell passivity in murine melanoma xenografts." dans: Ultrasound in medicine & biology, Vol. 40, Issue 9, pp. 2125-33, (2014) (PubMed).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF alpha (TNF alpha Produits)

Synonymes: anticorps DIF, anticorps TNF-alpha, anticorps TNFA, anticorps TNFSF2, anticorps RATTNF, anticorps Tnfa, anticorps tnf, anticorps TNF-a, anticorps TNFalpha, anticorps Tnfsf1a, anticorps TNFa, anticorps cTNF, anticorps Tnf-alpha, anticorps tnfa-like, anticorps TNF-ALPHA, anticorps dif, anticorps tnfa, anticorps xtnf, anticorps tnfsf2, anticorps tnf-alpha, anticorps Cachectin, anticorps tumor necrosis factor, anticorps tumor necrosis factor b (TNF superfamily, member 2), anticorps tumor necrosis factor alpha, anticorps tumor necrosis factor a (TNF superfamily, member 2), anticorps TNF, anticorps Tnf, anticorps tnf, anticorps tnfb, anticorps tnf-alpha, anticorps LOC103694380, anticorps tnfa

Sujet:

Synonyms: DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tnf

Background: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

ID gène: 21926

UniProt: P06804

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome