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Tumor Necrosis Factor anticorps (Tumor Necrosis Factor alpha) (AA 180-230) Primary Antibody

TNF Reactivité: Humain, Souris, Rat IF (p), IHC (p), WB Hôte: Lapin Polyclonal unconjugated
Pubmed (19) Independent Validation (1)
N° du produit ABIN677318
$339.90
Plus shipping costs $45.00
100 μL
local_shipping Destination: Etats-Unis
Envoi sous 4 à 6 jours ouvrables
  • Antigène
    Tumor Necrosis Factor (TNF)
    Épitope
    AA 180-230
    Reactivité
    Humain, Souris, Rat
    Hôte
    Lapin
    Clonalité
    Polyclonal
    Conjugué
    Cet anticorp Tumor Necrosis Factor est non-conjugé
    Application
    Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from mouse TNF-alpha
    Isotype
    IgG
  • Indications d'application
    WB 1:100-1000
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029732 (Immunofluorescence)
    'Independent Validation' signe
    by
    Confocal Imaging Core, Beth Israel Deaconess Medical Center
    No.
    #029732
    Date
    12.06.2014
    Antigène
    Numéro du lot
    140113
    Application validée
    Immunofluorescence
    Contrôle positif
    Normal mouse lung tissue
    Contrôle négative
    Normal mouse adipose tissue
    Conclusion
    Fluorescent signal is detected in positive control, but not in negative control or isotype controls.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antibody: Tumor Necrosis Factor (TNF)
    • Catalog number: ABIN677318
    • Lot number: 140113
    Anticorps secondaire
    • Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)
    • Lot number: Not available
    Full Protocol
    • Paraffin embedded positive and negative control tissue sections were deparaffinized and underwent antigen retrieval using 10 mM sodium citrate pH 6.0 in a pressure cooker for 10 min.
    • The tissue sections were incubated with 1 mg/mL sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
    • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
    • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
    • Tissue sections were rinsed three times in TBS for 5 min each at RT.
    • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
    • Tissue sections were rinsed three times in TBS for 5 min each at RT.
    • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen).
    • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
    Notes
    Nothing to note.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store at -20°C
    Date de péremption
    12 months
  • Lennikov, Mirabelli, Mukwaya, Schaupper, Thangavelu, Lachota, Ali, Jensen, Lagali: "Selective IKK2 inhibitor IMD0354 disrupts NF-κB signaling to suppress corneal inflammation and angiogenesis." dans: Angiogenesis, Vol. 21, Issue 2, pp. 267-285, 2019 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

    Han, Wang, Qin, Xia, Wu: "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." dans: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 83, 2018 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

    Wang, Tang, Shen, Chen, Chen, Luo, Ge: "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." dans: Cytokine, Vol. 99, pp. 275-280, 2018 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Mouse (Murine)).

    Li, Fang, Tian, Wang, Ao, Hou, Tong, Fan, Bai: "Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation." dans: Molecular medicine reports, Vol. 16, Issue 4, pp. 4455-4462, 2018 (PubMed). Méthode utilisée: Western Blotting (WB)

    Dong, Zhou, Ji, Pan, Zheng: "AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE-/- mice." dans: Biochemical and biophysical research communications, Vol. 489, Issue 4, pp. 426-431, 2017 (PubMed). Méthode utilisée: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Échantillon (espèces): Mouse (Murine)).

    Wang, Li, Liu, Wang, Cao: "Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin‑induced lung inflammation in a murine model of asthma." dans: Molecular medicine reports, Vol. 13, Issue 5, pp. 4289-302, 2017 (PubMed). Méthode utilisée: Western Blotting (WB)

    Shi, Yu, Liu, Liu, Zhang, Yang, Chai, Lou, Gao, Zhu: "Panax notoginseng saponins administration modulates pro- /anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats." dans: Metabolic brain disease, Vol. 32, Issue 1, pp. 221-233, 2016 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Rat (Rattus)). Détails: Sample: brain

    Ji, Wang, Wang, Chen, Li: "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits." dans: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 51-58, 2016 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Rabbit). Détails: Sample: lung

    Du, Niu, Wang, Zhao, Kong, Zhang, Nan: "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." dans: Molecular medicine reports, Vol. 13, Issue 3, pp. 2159-66, 2016 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Mouse (Murine)). Détails: Sample: liver

    Bertuglia, Pagliara, Grego, Ricci, Brkljaca-Bottegaro: "Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career." dans: BMC veterinary research, Vol. 12, Issue 1, pp. 246, 2016 (PubMed). Méthode utilisée: ELISA (ELISA)

    Wang, Li, Wei, Xu, Chen, Song, Lu, Wang: "Targeted knockout of TNF?? by injection of lentivirus?mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats." dans: Molecular medicine reports, Vol. 12, Issue 3, pp. 4389-95, 2015 (PubMed).

    Sun, Zeng, Cao, Song, Zhang, Liu: "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." dans: BioMed research international, Vol. 2015, pp. 919401, 2015 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Rat (Rattus)).

    Xu, Zhao, Lin, Wang, Hu, Jiang: "Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus." dans: BMC ophthalmology, Vol. 15, pp. 126, 2015 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Rat (Rattus)).

    Fang, Li, Sun, Bao, Ren, Lv, Ma: "Ischemic preconditioning protects against spinal cord ischemia-reperfusion injury in rabbits by attenuating blood spinal cord barrier disruption." dans: International journal of molecular sciences, Vol. 14, Issue 5, pp. 10343-54, 2015 (PubMed). Méthode utilisée: Western Blotting (WB)

    Luo, Wang, Li, Wei, Zhang, Liu, Wang, Niu, Wang: "Maternal bisphenol a diet induces anxiety-like behavior in female juvenile with neuroimmune activation." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 140, Issue 2, pp. 364-73, 2015 (PubMed). Méthode utilisée: Western Blotting (WB)

    Morishita, Oku, Horie, Tonari, Kida, Okubo, Sugiyama, Takai, Hara, Ikeda: "Systemic simvastatin rescues retinal ganglion cells from optic nerve injury possibly through suppression of astroglial NF-?B activation." dans: PLoS ONE, Vol. 9, Issue 1, pp. e84387, 2014 (PubMed). Méthode utilisée: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Échantillon (espèces): Rat (Rattus)).

    Wang, Hu, Wang, Gu, Gao, Cao, Zheng: "5-Aminolevulinic acid-mediated sonodynamic therapy reverses macrophage and dendritic cell passivity in murine melanoma xenografts." dans: Ultrasound in medicine & biology, Vol. 40, Issue 9, pp. 2125-33, 2014 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Mouse (Murine)).

    Wang, Zhang, Gu, Ding, Fan, Zhu, Shi, Hong, Kong: "Morin reduces hepatic inflammation-associated lipid accumulation in high fructose-fed rats via inhibiting sphingosine kinase 1/sphingosine 1-phosphate signaling pathway." dans: Biochemical pharmacology, Vol. 86, Issue 12, pp. 1791-804, 2013 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Rat (Rattus)). Détails: Sample: liver

  • Antigène
    Tumor Necrosis Factor (TNF)
    Autre désignation
    Tnf alpha (TNF Antibody Extrait)
    Synonymes
    DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa
    Sujet

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

    Subcellular location: Extracellular

    Synonyms: DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tnf

    ID gène
    21926
    UniProt
    P06804
    Pathways
    Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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