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Occludin anticorps (AA 431-522)

OCLN Reactivité: Humain, Souris, Porc WB, ELISA, FACS, IF (cc), IF (p), IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN687337
  • Antigène Voir toutes Occludin (OCLN) Anticorps
    Occludin (OCLN)
    Épitope
    • 16
    • 15
    • 15
    • 9
    • 7
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 431-522
    Reactivité
    • 104
    • 65
    • 46
    • 33
    • 18
    • 13
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    Humain, Souris, Porc
    Hôte
    • 86
    • 20
    • 3
    • 1
    Lapin
    Clonalité
    • 84
    • 26
    Polyclonal
    Conjugué
    • 46
    • 10
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp Occludin est non-conjugé
    Application
    • 88
    • 33
    • 28
    • 25
    • 25
    • 17
    • 15
    • 14
    • 12
    • 8
    • 7
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
     Réactivité croisée
    Humain, Souris, Porc
    Homologie
    Rat,Dog,Cow,Horse,Guinea Pig
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from mouse Occludin
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IF(IHC-P) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029632 (Immunofluorescence)
    'Independent Validation' signe
    by
    Confocal Imaging Core, Beth Israel Deaconess Medical Center
    No.
    #029632
    Date
    23.03.2014
    Antigène
    Numéro du lot
    990698W
    Application validée
    Immunofluorescence
    Contrôle positif
    Mouse stomach tissue
    Contrôle négative
    Human fat tissue
    Conclusion
    Signal was detected in positive control sample and not in negative control sample.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antibody: Occludin (OCLN)
    • Catalog number: ABIN687337
    • Lot number: 990698W
    Anticorps secondaire
    • Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)
    Full Protocol
    • Paraffin embedded positive and negative control tissue sections were deparaffinized and undergone antigen retrieval using 10 mM Sodium Citrate pH6 in a pressure cooker for 10 min.
    • The tissue sections were incubated with 1 mg/mL Sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
    • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
    • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
    • Tissue sections were rinsed three times in TBS for 5 min each at RT.
    • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
    • Tissue sections were rinsed three times in TBS for 5 min each at RT.
    • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen)
    • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
    Notes
    • No challenges notes
    • OCLN is recommended to staining of mouse stomach tissue according to this protocol.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Sun, Liu, Xue, Wang: "Effects of insulin combined with idebenone on blood-brain barrier permeability in diabetic rats." dans: Journal of neuroscience research, Vol. 93, Issue 4, pp. 666-77, (2015) (PubMed).

    Zhao, Qin, Han, Wang, Zhang, Liu: "?-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2." dans: International journal of molecular sciences, Vol. 15, Issue 2, pp. 1915-26, (2014) (PubMed).

    Pan, Qin, Zhao, Wang, Liu, Che: "Effects of soybean agglutinin on mechanical barrier function and tight junction protein expression in intestinal epithelial cells from piglets." dans: International journal of molecular sciences, Vol. 14, Issue 11, pp. 21689-704, (2013) (PubMed).

    Zhao, Qin, Sun, Che, Bao, Zhang: "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets." dans: International journal of molecular sciences, Vol. 12, Issue 12, pp. 8502-12, (2012) (PubMed).

    Gu, Xue, Wei, Zhang, Li: "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." dans: Neuroscience letters, Vol. 493, Issue 3, pp. 140-4, (2011) (PubMed).

  • Antigène
    Occludin (OCLN)
    Autre désignation
    Occludin (OCLN Produits)
    Synonymes
    anticorps AI503564, anticorps Ocl, anticorps ocln, anticorps oclnb, anticorps tpmt, anticorps OCLN, anticorps wu:fd23h10, anticorps wu:fi13c01, anticorps zgc:113992, anticorps zgc:56359, anticorps BLCPMG, anticorps occludin, anticorps occludin S homeolog, anticorps occludin a, anticorps Ocln, anticorps OCLN, anticorps ocln.S, anticorps ocln, anticorps oclna
    Sujet

    Synonyms: Ocl, AI53564, Occludin, Ocln

    Background: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

    ID gène
    18260
    UniProt
    Q61146
    Pathways
    Cell-Cell Junction Organization, Hepatitis C
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