Immunofluorescence (1:10-50) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Gloyn, Noordam, Willemsen, Ellard, Lam, Campbell, Midgley, Shiota, Buettger, Magnuson, Matschinsky, Hattersley: "Insights into the biochemical and genetic basis of glucokinase activation from naturally occurring hypoglycemia mutations." dans: Diabetes, Vol. 52, Issue 9, pp. 2433-40, (2003) (PubMed).
Pruhova, Ek, Lebl, Sumnik, Saudek, Andel, Pedersen, Hansen: "Genetic epidemiology of MODY in the Czech republic: new mutations in the MODY genes HNF-4alpha, GCK and HNF-1alpha." dans: Diabetologia, Vol. 46, Issue 2, pp. 291-5, (2003) (PubMed).
Rizzo, Magnuson, Drain, Piston: "A functional link between glucokinase binding to insulin granules and conformational alterations in response to glucose and insulin." dans: The Journal of biological chemistry, Vol. 277, Issue 37, pp. 34168-75, (2002) (PubMed).