TIGIT anticorps (AA 22-141)

N° du produit ABIN6939305

Détail du produit anti-TIGIT anticorps

Antigène: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Épitope: AA 22-141

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TIGIT est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Coating (Coat), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment (around aa 22-141) of human TIGIT protein (exact sequence is proprietary)

Clone: TIGIT-3017

Isotype: IgG2c kappa

Information d'application

Indications d'application:

Positive Control: MOLT-4, Prostate or Colon Carcinoma.

Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur TIGIT

Antigène: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Autre désignation: TIGIT (TIGIT Produits)

Sujet: TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation). It has been demonstrated to be upregulated on T cells in many cancers and is an immuno-oncology target for therapy.

Poids moléculaire: 26kDa

ID gène: 201633

Pathways: Cancer Immune Checkpoints