Smooth Muscle Actin anticorps

N° du produit ABIN6940520

Détail du produit anti-Smooth Muscle Actin anticorps

Antigène: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Smooth Muscle Actin est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Specificité: Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Réactivité croisée (Details): Predicted to show a broad reactivity.

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human ACTA2 protein

Clone: ACTA2-791

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Smooth Muscle Actin

Antigène: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Autre désignation: ACTA2 (ACTA2 Produits)

Synonymes: anticorps AAT6, anticorps ACTSA, anticorps MYMY5, anticorps GAMMA-ACTIN, anticorps ACT, anticorps ACTA3, anticorps ACTE, anticorps ACTL3, anticorps ACTSG, anticorps Act-4, anticorps Act4, anticorps SMGA, anticorps 0610041G09Rik, anticorps Actvs, anticorps SMalphaA, anticorps a-SMA, anticorps alphaSMA, anticorps wu:fb63d03, anticorps ASMA, anticorps SMactin, anticorps a-sma, anticorps aat6, anticorps actsa, anticorps mymy5, anticorps sm-actin, anticorps ACTA2, anticorps actc1, anticorps ACT-4, anticorps asma, anticorps ACTGE, anticorps actin, alpha 2, smooth muscle, aorta, anticorps actin, gamma 2, smooth muscle, enteric, anticorps actin, alpha 2, smooth muscle, aorta L homeolog, anticorps ACTA2, anticorps ACTG2, anticorps Actg2, anticorps Acta2, anticorps acta2, anticorps acta2.L

Poids moléculaire: 42kDa

ID gène: 59

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development