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VISTA anticorps

VISTA Reactivité: Humain ELISA, IHC, Coat, StM Hôte: Souris Monoclonal VISTA-3007 unconjugated
N° du produit ABIN6940575
  • Antigène Voir toutes VISTA Anticorps
    VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
    Reactivité
    • 35
    • 17
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 30
    • 11
    • 5
    • 2
    • 1
    • 1
    Souris
    Clonalité
    • 29
    • 20
    • 2
    Monoclonal
    Conjugué
    • 26
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp VISTA est non-conjugé
    Application
    • 30
    • 21
    • 16
    • 14
    • 8
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)
    Purification
    Purified by Protein A/G
    Immunogène
    Recombinant full-length human VISTA protein
    Clone
    VISTA-3007
    Isotype
    IgG2b kappa
  • Indications d'application

    Positive Control: Spleen or Placenta.

    Known Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
    Autre désignation
    VSIR (VISTA Produits)
    Synonymes
    anticorps MGC112715, anticorps MGC151567, anticorps B7-H5, anticorps B7H5, anticorps GI24, anticorps PP2135, anticorps SISP1, anticorps Dies1, anticorps VISTA, anticorps V-set immunoregulatory receptor, anticorps vsir, anticorps Vsir, anticorps VSIR
    Sujet
    VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC s, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy.
    Poids moléculaire
    34kDa
    ID gène
    64115
    UniProt
    Q9H7M9
    Pathways
    Cancer Immune Checkpoints
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