Recombinant GLUT1 anticorps (AA 203-305)

N° du produit ABIN6940597

Détail du produit anti-GLUT1 anticorps

Antigène: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Type d'anticorp: Recombinant Antibody

Épitope: AA 203-305

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp GLUT1 est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Coating (Coat), Staining Methods (StM)

Specificité: Recognizes a protein of 55 kDa, which is identified as GLUT-1. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut-1 to Glut-12. Glut-1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut-1 is expressed at variable levels in many human tissues. Overexpression of Glut-1 has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut-1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative).

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment of human GLUT1 protein (around aa 203-305) (exact sequence is proprietary)

Clone: GLUT1-3132R

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: K562, A431, MDA-MB-231 cells. Erythrocytes. Mesothelioma or breast, colon and ovarian carcinoma.

Known Application: ELISA (For coating use Ab at 1-2 μg/mL order Ab without BSA), Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur GLUT1

Antigène: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Autre désignation: SLC2A1 (SLC2A1 Produits)

Poids moléculaire: 55kDa

ID gène: 6513

UniProt: P11166

Pathways: Sensory Perception of Sound, Dicarboxylic Acid Transport, L'effet Warburg