OGT anticorps

N° du produit ABIN6944354

Détail du produit anti-OGT anticorps

Antigène: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp OGT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by Protein A.

Immunogène: Recombinant protein within Human OGT aa 1000 to the C-terminus

Clone: 11F2

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 12 months

Détails sur OGT

Antigène: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Autre désignation: OGT (OGT Produits)

Synonymes: anticorps BcDNA:GH04245, anticorps CG10392, anticorps Dmel\\CG10392, anticorps OGT, anticorps Ogt, anticorps P1201, anticorps SXC, anticorps Sxc, anticorps l(2)02637, anticorps l(2)NC130, anticorps sxc/Ogt, anticorps fm81g08, anticorps ogt, anticorps wu:fc12b01, anticorps wu:fm81g08, anticorps HRNT1, anticorps O-GLCNAC, anticorps 1110038P24Rik, anticorps 4831420N21Rik, anticorps AI115525, anticorps Ogtl, anticorps O-linked N-acetylglucosamine (GlcNAc) transferase L homeolog, anticorps super sex combs, anticorps O-linked N-acetylglucosamine (GlcNAc) transferase, tandem duplicate 1, anticorps O-linked N-acetylglucosamine (GlcNAc) transferase, anticorps O-linked GlcNAc transferase, anticorps o-linked GlcNAc transferase, anticorps O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), anticorps ogt.L, anticorps sxc, anticorps ogt.1, anticorps OGT, anticorps ogt, anticorps GL50803_12081, anticorps GL50803_41701, anticorps THAPSDRAFT_264441, anticorps spyA, anticorps Ogt

Sujet:

Synonyms: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit, OGT, OGT1, HRNT1, MRX106, HINCUT-1, O-GLCNAC.

Background: Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. O-GlcNAc transferase has been purified from rat liver. It exists as a heterotrimeric complex with two subunits of the same molecular mass and one shorter subunit. Both polypeptides are related, the short subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.

ID gène: 8473

UniProt: O15294

Pathways: Regulation of Carbohydrate Metabolic Process