5-Methylcytosine anticorps

N° du produit ABIN6971343

Détail du produit

Antigène: 5-Methylcytosine

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Methylated DNA Immunoprecipitation (MeDIP), Immunofluorescence (IF), Dot Blot (DB)

Attributs du produit: 5-Methylcytosine (5-mC) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone A1) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis. 5-Methylcytosine (5-mC) antibody (mAb) (Clone A1) was raised in a Mouse host. It has been validated for use in Dot blot, Immunofluorescence and Methyl DNA Immunoprecipitation, it has been shown to react with Human samples, but the sequence is not species specific so it should react with a wide range of sample types.

Purification: Protein A Chromatography

Immunogène: This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.

Clone: A1

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détail du antigène

Antigène: 5-Methylcytosine

Autre désignation: 5-Methylcytosine (5-mC)

Classe de substances: Chemical