PHLPP1 anticorps (N-Term)

N° du produit ABIN6991746

Détail du produit anti-PHLPP1 anticorps

Antigène: PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))

Épitope: AA 250-300, N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PHLPP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: At least four isoforms are known to exist, this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.

Purification: PHLPP1 Antibody is affinity chromatography purified via peptide column.

Immunogène: PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PHLPP1. The immunogen is located within amino acids 250 - 300 of PHLPP1.

Isotype: IgG

Information d'application

Indications d'application: PHLPP1 antibody can be used for detection of PHLPP1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PHLPP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: PHLPP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur PHLPP1

Antigène: PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))

Autre désignation: PHLPP1 (PHLPP1 Produits)

Synonymes: anticorps PHLPP, anticorps PLEKHE1, anticorps SCOP, anticorps AI836256, anticorps Phlpp, anticorps Plekhe1, anticorps mKIAA0606, anticorps Scop, anticorps PH domain and leucine rich repeat protein phosphatase 1, anticorps PHLPP1, anticorps phlpp1, anticorps Phlpp1

Sujet: PHLPP1 Antibody: PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.

Poids moléculaire: 189 kDa

ID gène: 23239

NCBI Accession: NP_919431

UniProt: O60346

Pathways: Signalisation PI3K-Akt, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway