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TNFSF9 anticorps (AA 101-200)

TNFSF9 Reactivité: Humain, Souris, Rat WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN705056
  • Antigène Voir toutes TNFSF9 Anticorps
    TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))
    Épitope
    • 14
    • 8
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 101-200
    Reactivité
    Humain, Souris, Rat
    Hôte
    • 41
    • 20
    • 10
    • 2
    Lapin
    Clonalité
    • 36
    • 34
    • 2
    Polyclonal
    Conjugué
    • 40
    • 6
    • 5
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp TNFSF9 est non-conjugé
    Application
    • 47
    • 37
    • 30
    • 12
    • 12
    • 8
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
     Réactivité croisée
    Humain, Souris, Rat
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human TNFSF9
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Restrictions
    For Research Use only
  • Validation #029578 (Immunohistochemistry)
    'Independent Validation' signe
    by
    Immunohistochemistry Core, NYU Langone
    No.
    #029578
    Date
    18.01.2014
    Antigène
    Numéro du lot
    999892W
    Application validée
    Immunohistochemistry
    Contrôle positif
    Human liver
    Contrôle négative
    Human placental decidual cells
    Conclusion
    Signal was detected in positive control tissue and not in negative control tissue.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antibody: Tumor necrosis factor ligand superfamily member 9 (TNFSF9)
    • Catalog number: ABIN705056
    • Lot number: 999892W
    Anticorps secondaire
    • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
    • Lot number: D05923BA
    Full Protocol
    • Immunohistochemistry was performed on a Ventana NexES automated platform, instrument manufacturer specific reagents are italicized.
    • 1. Slides were preheated in convection oven at 60°C for 30 minutes
    • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 minutes each - 3 changes of 100% Ethanol, 3 minutes each - 3 changes of 95% Ethanol, 3 minutes each - Rinsed in distilled water, 3 changes
    • 1. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 minutes. - Slides were allowed to cool (in citrate) for 30 minutes. - Slides were washed x 3 in Distilled water
    • 1. NexES instrument procedure, iVIEW DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
    • 1. Slides rinsed with *reaction buffer* x 3
    • 1. *iVIEW Inhibitor (H2O2)* applied and incubated for 4 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliters applied/slide) - Ventana Isotype control applied neat - Slides incubated overnight at room temperature (~12 hours ~25°C)
    • 1. Slides rinsed with *reaction buffer* x3
    • 1. *iVIEW Biotinylated IgG* applied and incubated for 8 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. *iVIEW Streptavidin-Horseradish Peroxidase* applied and incubated for 8 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. *iVIEW DAB/H2O2* applied and incubated for 8 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. *iVIEW Copper* applied and incubated for 4 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. Slides washed in Dawn Detergent/tap water
    • 1. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 seconds - Slides washed in tap water, 1 minute - Decolorized (10% Acetic Acid in 70% ethanol), 1 minute - Slides washed in tap water, 1 minute - Bluing (Austin Clear Ammonia), 1 minute - Slides washed in tap water, 1 minute
    • 1. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 minutes each - 3 changes of 100% Ethanol, 3 minutes each - 3 changes of Xylene, 5 minutes each - Mounted with Permount
    • 1. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
    Notes
    • Deviations from protocol/procedure supplied by manufacturer:
    • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
    • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
    • Step 3.1: Slides heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
    • Step 3.2: Slides cooled for 30 minutes; manufacturer cools for 20 minutes.
    • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
    • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
    • Step 8.1: Antibody diluted in PBS at 1:250; manufacture recommends dilution range (1:100-1:500). No primary antibody diluent recommended.
    • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
    • Tissue Interpretation (limited): • TNFSF9: Under the staining parameters described above, placenta was weakly cytoplasmic positive (trophoblastic and decidua cells). Liver hepatocytes were weakly stained. Substantial signal detected in limited number of other tissues, including kidney, prostate, and skin.
    • • I-NC (Isotype negative control): No signal detected
    • • B-NC (Blank negative control): No signal detected
    • Signal Localization: - Signal to noise was adequate with predominately cytoplasmic subcellular localization observed. Nuclear staining was rare and no distinct membrane signal was detected.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Antigène
    TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))
    Autre désignation
    TNFSF9/CD137L (TNFSF9 Produits)
    Synonymes
    anticorps TNFSF9, anticorps 4-1BB-L, anticorps 4-1BBL, anticorps AI848817, anticorps Cd137l, anticorps Ly63l, anticorps CD137L, anticorps TNF superfamily member 9, anticorps tumor necrosis factor (ligand) superfamily, member 9, anticorps Tnfsf9, anticorps LOC476729, anticorps TNFSF9
    Sujet

    Synonyms: CD137L, 4-1BB-L, Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, TNFSF9

    Background: Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.

    ID gène
    8744
    UniProt
    P41273
    Pathways
    Activated T Cell Proliferation, Cancer Immune Checkpoints
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