RIPK1 anticorps (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1)

Details for Product anti-RIPK1 Antibody No. ABIN715016
Antigène
  • RIP
  • RIP1
  • D330015H01Rik
  • Rinp
  • Rip1
  • rip1
  • ripk1
  • xRIP1beta
  • xrip1
  • receptor interacting serine/threonine kinase 1
  • receptor (TNFRSF)-interacting serine-threonine kinase 1
  • receptor interacting serine/threonine kinase 1 L homeolog
  • RIPK1
  • Ripk1
  • ripk1.L
Reactivité
Humain, Souris, Rat (Rattus)
165
57
46
8
4
3
3
1
1
Hôte
Lapin
137
31
3
Clonalité
Polyclonal
Conjugué
Cet anticorp RIPK1 est non-conjugé
10
6
6
6
5
5
5
5
5
3
2
2
2
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
113
56
55
27
23
15
5
4
4
2
2
2
1
Options
Immunogène KLH conjugated synthetic peptide derived from human RIPK-1/RIP
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others RIPK1 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Ripk-1 (RIPK1 Antibody Extrait)
Sujet

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

Subcellular location: Cytoplasm, Cell membrane

Synonyms: RIP, RIP1, Receptor-interacting serine/threonine-protein kinase 1, Cell death protein RIP, Receptor-interacting protein 1, RIP-1, Serine/threonine-protein kinase RIP, RIPK1

ID gène 8737
UniProt Q13546
Pathways Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling
Indications d'application WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20°C
Date de péremption 12 months
Produit citée dans: Seifert, Werba, Tiwari, Giao Ly, Alothman, Alqunaibit, Avanzi, Barilla, Daley, Greco, Torres-Hernandez, Pergamo, Ochi, Zambirinis, Pansari, Rendon, Tippens, Hundeyin, Mani, Hajdu, Engle, Miller: "The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression." dans: Nature, Vol. 532, Issue 7598, pp. 245-9, 2016 (PubMed).

Luo, Roy, Xiao, Sun, Liang, Chen, Fu, Sun, Zhu, Ye, Liu: "Lycorine induces programmed necrosis in the multiple myeloma cell line ARH-77." dans: Tumour biology, 2014 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

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