Elastin (ELN) (AA 750-786) anticorps

Détails pour le produit réf. ABIN734003, Fournisseur: Connectez-vous pour afficher
Antigène
  • SVAS
  • WBS
  • WS
  • AI385707
  • AI480567
  • E030024M20Rik
  • RATTREL11
  • TREL11
  • Trela
  • Trela26
  • ELN
  • elastin
  • ELN
  • Eln
  • LOC574132
  • Tcur_2481
  • Kfla_2289
  • LOC100620140
Épitope
AA 750-786
8
6
5
2
2
2
2
1
Reactivité
Humain, Souris, Rat (Rattus)
67
21
17
15
13
12
5
4
3
3
3
3
1
Hôte
Lapin
57
29
2
1
Clonalité
Polyclonal
Conjugué
Inconjugué
4
3
2
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
57
40
29
26
12
10
10
9
8
3
2
2
1
Options
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Supplier Product No.
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'Independent Validation' signe
Numéro du lot 120306
Application validée Immunohistochemistry
Contrôle positif Mouse heart tissue
Contrôle négative Mouse adipose tissue
Conclusion Signal was detected in positive control tissue and not detected in negative control tissue.
Anticorps primaire
  • Antibody: Elastin (ELN) (C-Term) antibody
  • Catalog number: ABIN734003
  • Lot number: 120306
Contrôle isotypique
Anticorps secondaire
Contrôles
  • Positive control: Wild type mouse heart tissue (specimen known to contain the target protein).
  • Negative control: Wild type adipose tissue (specimen known to not contain the target protein).
  • Primary antibody isotype control: Wild type mouse heart treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Wild type mouse heart treated with secondary antibody only (no
  • primary antibody).
Protocole
  • Immunohistochemistry was performed manually.
  • Sections were deparaffinized and rehydrated.
  • Sections were heated to 98°C for 10 min in 10 mM citrate buffer pH 6.0 for antigen retrieval.
  • Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidase.
  • Sections were washed x 3 in Tris buffered saline (TBS).
  • Sections were blocked in 5% normal donkey serum for 60 min at room temperature.
  • Sections were incubated with primary antibody diluted 1:1000 in 5% normal donkey serum in TBS. Incubated at 4°C overnight.
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature.
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
  • Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 seconds at room temperature.
  • Sections were washed x 3 in Distilled Water.
  • Sections were counterstained with hematoxylin for 1 min.
  • Sections were washed x 1 in Distilled Water.
  • Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.
Notes None
Validation Images
Immunohistochemistry validation image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Figure 1: Immunostaining of Elastin antibody. Panel 1: Staining of mouse heart tissue...
Immunogène KLH conjugated synthetic peptide derived from human Elastin
Isotype IgG
Purification Purified by Protein A.
Autre désignation Elastin (ELN Antibody Extrait)
Sujet

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).

Subcellular location: Extracellular, Secreted

Synonyms: WS, WBS, SVAS, Elastin, Tropoelastin, ELN
ID gène 2006
UniProt P15502
Pathways Regulation of Actin Filament Polymerization
Indications d'application WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20°C
Date de péremption 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Formalin-fixed and paraffin embedded human artery labeled with Anti-Elastin Polyclona...
Immunohistochemistry (IHC) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Formalin-fixed and paraffin embedded human glioma labeled with Anti-Elastin Polyclona...
SDS-PAGE (SDS) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) L1 mouse intestine lysates L2 rat lung lysates probed with Anti Alpha Elastin Polyclo...
Immunohistochemistry (IHC) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Formalin-fixed and paraffin embedded rat intervertebral disk labeled with Anti-ELASTI...
Immunohistochemistry (IHC) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Images provided the Independent Validation Program (badge number 28751): Formalin-fix...
Flow Cytometry (FACS) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Human A549 cells probed with Rabbit Anti-Elastin Polyclonal Antibody
Western Blotting (WB) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Mouse lung lysates probed with Elastin Polyclonal Antibody, unconjugated at 1:300 ov...
Produit citée dans: Hasegawa, Sato, Shirai, Watanabe, Yamamoto, Watanabe, Nohtomi, Hirano, Watanabe: "Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo." dans: PLoS ONE, Vol. 9, Issue 12, pp. e110866, 2014 (PubMed).

Konii, Sato, Kikuchi, Okiyama, Watanabe, Hasegawa, Yamamoto, Itoh, Hirano, Watanabe: "Stimulatory effects of cardiotrophin 1 on atherosclerosis." dans: Hypertension, Vol. 62, Issue 5, pp. 942-50, 2013 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Guo, Li, Dong, Chen, Deng, Wang, Ying: "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." dans: Colloids and surfaces. B, Biointerfaces, Vol. 96, pp. 29-36, 2012 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Mouse (Murine)).

Ikeda, Yada, Takase: "[Syntheses of 1,3,4-thiadiazole derivatives]." dans: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, Vol. 90, Issue 1, pp. 95-8, 1970 (PubMed).

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