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PD-1 anticorps (AA 201-288)

PDCD1 Reactivité: Humain, Souris, Rat FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN735608
  • Antigène Voir toutes PD-1 (PDCD1) Anticorps
    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
    Épitope
    • 15
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 201-288
    Reactivité
    • 145
    • 85
    • 24
    • 5
    • 3
    • 1
    Humain, Souris, Rat
    Hôte
    • 75
    • 74
    • 35
    • 12
    • 9
    • 3
    • 2
    Lapin
    Clonalité
    • 152
    • 55
    • 2
    Polyclonal
    Conjugué
    • 100
    • 16
    • 13
    • 11
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp PD-1 est non-conjugé
    Application
    • 132
    • 59
    • 45
    • 40
    • 38
    • 18
    • 13
    • 12
    • 12
    • 11
    • 10
    • 10
    • 10
    • 9
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))
     Réactivité croisée
    Humain, Souris, Rat
    Homologie
    Cow,Horse,Rabbit
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human PD-1
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #104535 (Immunohistochemistry)
    'Independent Validation' signe
    by
    TRON
    No.
    #104535
    Date
    29.11.2023
    Antigène
    PD-1
    Numéro du lot
    BC04411027
    Application validée
    Immunohistochemistry
    Contrôle positif

    FFPE human tonsil

    Contrôle négative
    Conclusion

    Passed. The PD-1 antibody (AA 201-288) ABIN735608 specifically stains cells within the germinal zone in human tonsil.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN735608
    Anticorps secondaire
    HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122)
    Full Protocol
    • Dewax and rehydrate tissue sections on glass slides in a descending xylene and graded alcohol series:
      • xylene 2x for 5 min.
      • 100 % (v/v) ethanol 2x for 5 min.
      • 96 % (v/v) ethanol 2x for 5 min.
      • 70 % (v/v) ethanol 2x for 5 min.
    • Wash slides 2x 5 min with ddH2O.
    • Cook slides for 30 min at 95 °C in citrate buffer pH 6.
    • Wash slides 2x 3 min with ddH2O.
    • Incubate slides in 0.3 % (v/v) H2O2 in PBS for 15 min to quench endogenous peroxidase.
    • Wash slides 3x 3 min in 1X PBS.
    • Block slides in 10 % goat serum in PBS for 30 min at RT.
    • Incubate slides with primary rabbit anti-PD-1 antibody (antibodies-online, ABIN735608, lot BC04411027) diluted 1:250 and 1:500 in 10 % goat serum in PBS ON at 4 °C in a humidified chamber.
    • Wash slides 3x 3 min in 1X PBS.
    • Incubate slides with secondary HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122) ready-to-use in 10 % goat serum in PBS for 30 min at RT in a humidified chamber.
    • Wash slides 3x 3 min in 1X PBS.
    • HRP development.
    • Counterstain slides with hematoxylin solution (Carl Roth, T865.2).
    • Wash slides for 15 min under running tap water.
    • Dehydrate sections in an ascending alcohol-xylene series:
      • 70% ethanol 2x for 10 sec.
      • 96% ethanol 2x for 10 sec.
      • 100% ethanol 2x for 10 sec.
      • xylene 2x for 10 sec.
    • Mount sections with Entellan new mounting medium (Sigma-Aldrich, 1008690500).
    • Dry sections.
    • Image acquisition on an Aperio CS2 Digital Pathology Slide Scanner (Leica), Magnification 40x.
    Notes
    • Staining with ABIN735608 the signal at 1:250 and 1:500 antibody dilution in human tonsil was sharp and localized in the cells within the germinal zone (as expected).

    • ABIN735608 reactivity was also tested in mouse samples. However, in mouse spleen, the signal was almost absent and very weak at Ab dilution 1:250 and 1:500. No signal was detected in mouse pancreas. Even increasing the Ab concentration to 1:150 dilution, in mouse tumor tissue, ABIN735608 detected a very weak, unclear, and sporadic signal. The quality of the signal does not allow the possibility of background staining to be excluded.

  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Zhang, Niyazi, Hong, Tuluwengjiang, Zhang, Zhang, Su, Bao: "Effect of EBI3 on radiation-induced immunosuppression of cervical cancer HeLa cells by regulating Treg cells through PD-1/PD-L1 pathway." dans: Tumour biology, Vol. 39, Issue 3, pp. 1010428317692240, (2017) (PubMed).

    Szabó, Gáspár, Dajnoki, Papp, Fábos, Szegedi, Zeher: "Expansion of circulating follicular T helper cells associates with disease severity in childhood atopic dermatitis." dans: Immunology letters, Vol. 189, pp. 101-108, (2017) (PubMed).

  • Antigène
    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
    Autre désignation
    PD-1/CD279 (PDCD1 Produits)
    Synonymes
    anticorps CD279, anticorps PD-1, anticorps PD1, anticorps SLEB2, anticorps hPD-1, anticorps hPD-l, anticorps Ly101, anticorps Pdc1, anticorps programmed cell death 1, anticorps PDCD1, anticorps Pdcd1
    Sujet

    Synonyms: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, Programmed cell death protein 1, Protein PD-1, PDCD1

    Background: Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.

    ID gène
    5133
    UniProt
    Q15116
    Pathways
    Cancer Immune Checkpoints
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