GDNF anticorps (Glial Cell Line Derived Neurotrophic Factor) (AA 166-211)

Details for Product anti-GDNF Antibody No. ABIN736536
Antigène
  • gdnf
  • GDNF
  • AI385739
  • gdnfa
  • hfb1-gdnf
  • ATF1
  • ATF2
  • HFB1-GDNF
  • HSCR3
  • glial cell derived neurotrophic factor
  • glial cell derived neurotrophic factor a
  • glial cell line derived neurotrophic factor
  • glial cell derived neurotrophic factor L homeolog
  • Gdnf
  • gdnfa
  • GDNF
  • gdnf.L
Épitope
AA 166-211
14
14
9
9
6
5
4
3
2
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
171
87
60
1
Hôte
Lapin
165
10
8
4
2
Clonalité
Polyclonal
Conjugué
Cet anticorp GDNF est non-conjugé
19
9
6
5
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
161
62
47
33
26
26
12
12
11
6
6
4
3
2
1
1
Options
'Independent Validation' signe
Antigène GDNF
Numéro du lot 9F01M8
Application validée Immunofluorescence
Contrôle positif mouse hippocampus and putamen
Contrôle négative no primary control
Conclusion

ABIN736536 works in IF albeit with some background staining that we have been unable to eliminate although using different dilutions to increase the signal-to-noise ratio.

Anticorps primaire ABIN736536
Anticorps secondaire anti-rabbit AF488 conjugated antibody (Life Technologies)
Protocole
  • Perfuse mouse with 4% paraformaldehyde in 0.1M phosphate buffer (PB) pH7.4.
  • Post-fix spinal cord and brain blocks in the same fixative for additional 2h at RT.
  • Wash spinal cord and brain blocks several times with PBS.
  • Cut blocks with a vibratome (Leica, VT1000 S) into 70µm thick transverse sections.
  • Cut dorsal root ganglions (DRGs) with a cryostat into 17µm thick sections after cryoprotection and glass mounting.
  • Block free floating vibratome and glass mounted cryostat sections with blocking solution (0.01M PBS5% Normal Goat Serum (NGS; Sigma, G9023, lot SLBV1396), 0.1% Triton X-100 (BioRad, 161-0407, lot 00583) for 1h at RT.
  • Incubate sections with primary rabbit anti-GDNF antibody (antibodies-online, ABIN736536, lot 9F01M8) diluted 1:100 in blocking solution ON at RT.
  • Wash sections 4x for 5min with 0.01M PBS.
  • Incubate sections with secondary goat anti-rabbit AF488 conjugated antibody (Life Technologies) diluted 1:500 in PBS for 1h at RT.
  • Wash sections 4x for 5min with 0.01M PBS.
  • Mount sections in Fluoroshield (Sigma, F6182, lot MKCB0153V).
Notes
  • Different dilutions were also tested (1:200, 1:500, 1:2000) with or without Triton-X but the 1:100 dilution and the use of Triton-X in the blocking solution gave the best results.

  • Staining is mainly present in the cytoplasm of small-size neurons in the DRG, consistent with what has been previously described. ABIN736536 stains fibers in superficial laminae of the dorsal horn of the spinal cord. In the hippocampus staining is present in the CA1 both in the pyramidal neurons (cell body and dendrites) and in individual interneurons. In the putamen ABIN736536 stains neuron cell bodies and some dendrites.

Validation Images
Immunofluorescence validation image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) IF staining with ABIN736536 of mouse dorsal root ganglion (DRG, A), the dorsal horn o...
Immunogène KLH conjugated synthetic peptide derived from human GDNF
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others GDNF products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation GDNF (GDNF Antibody Extrait)
Sujet

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Subcellular location: Secreted

Synonyms: ATF1, ATF2, HSCR3, HFB1-GDNF, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

ID gène 2668
UniProt P39905
Pathways Signalisation RTK, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
Indications d'application WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20°C
Date de péremption 12 months
Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Anti...
Immunohistochemistry (IHC) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-GDNF Polyc...
SDS-PAGE (SDS) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti GDNF Polyclonal ...
Western Blotting (WB) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Lane 1:U251 lysates and Lane 2: Mouse brain lysates probed with Rabbit Anti-GDNF Poly...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Anti...
Produit citée dans: Zhang, Cai, Song, Dong, Hou, Lv: "Normalization of ventral tegmental area structure following acupuncture in a rat model of heroin relapse." dans: Neural regeneration research, Vol. 9, Issue 3, pp. 301-7, 2014 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Rat (Rattus)). Détails: Sample: Brain

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