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MTOR anticorps (pSer2448)

mTOR Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poisson WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN747158
  • Antigène Voir toutes MTOR (mTOR) Anticorps
    MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
    Épitope
    • 33
    • 28
    • 23
    • 15
    • 12
    • 11
    • 9
    • 7
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer2448
    Reactivité
    • 213
    • 139
    • 120
    • 22
    • 16
    • 12
    • 7
    • 6
    • 6
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Boeuf (Vache), Poisson
    Hôte
    • 193
    • 16
    • 2
    • 2
    Lapin
    Clonalité
    • 173
    • 39
    Polyclonal
    Conjugué
    • 109
    • 11
    • 10
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp MTOR est non-conjugé
    Application
    • 147
    • 64
    • 62
    • 51
    • 51
    • 44
    • 36
    • 35
    • 17
    • 17
    • 11
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificité
    This phosphorylation site is homologous across the listed species.
     Réactivité croisée
    Boeuf (Vache), Poisson, Humain, Souris, Rat
    Homologie
    Dog,Cow,Sheep,Horse,Rabbit
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Ser2448
    Isotype
    IgG
    Top Product
    Discover our top product mTOR Anticorps primaire
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Gugel, Ebner, Grimm, Czemmel, Paulsen, Hagel, Tatagiba, Nahnsen, Tabatabai: "Contribution of mTOR and PTEN to Radioresistance in Sporadic and NF2-Associated Vestibular Schwannomas: A Microarray and Pathway Analysis." dans: Cancers, Vol. 12, Issue 1, (2020) (PubMed).

    Mei, Xiang, Mei, Fang, Wang, Cao, Hu, Guo: "Pterostilbene inhibits nutrient metabolism and induces apoptosis through AMPK activation in multiple myeloma cells." dans: International journal of molecular medicine, Vol. 42, Issue 5, pp. 2676-2688, (2019) (PubMed).

    Qiu, Li, He, Sun, Li, Xin: "Distinct subgroup of the Ras family member 3 (DIRAS3) expression impairs metastasis and induces autophagy of gastric cancer cells in mice." dans: Journal of cancer research and clinical oncology, Vol. 144, Issue 10, pp. 1869-1886, (2018) (PubMed).

    Yang, Wang, Wang, Zhang, Zhang, Lu, Wang: "mTOR is involved in 17?-estradiol-induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." dans: Molecular reproduction and development, Vol. 82, Issue 4, pp. 305-14, (2015) (PubMed).

    Lin, Lin, Kang, Liu, Wang, Zheng, Yu, Lin: "Similar PDK1-AKT-mTOR pathway activation in balloon cells and dysmorphic neurons of type II focal cortical dysplasia with refractory epilepsy." dans: Epilepsy research, Vol. 112, pp. 137-49, (2015) (PubMed).

  • Antigène
    MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
    Autre désignation
    mTOR (mTOR Produits)
    Synonymes
    anticorps FRAP1, anticorps FRAP, anticorps FRAP2, anticorps RAFT1, anticorps RAPT1, anticorps 2610315D21Rik, anticorps AI327068, anticorps Frap1, anticorps flat, anticorps frap1, anticorps tor, anticorps wu:fc22h08, anticorps mechanistic target of rapamycin kinase, anticorps mechanistic target of rapamycin, anticorps mechanistic target of rapamycin (serine/threonine kinase), anticorps MTOR, anticorps Mtor, anticorps mtor
    Sujet

    Synonyms: FRAP, FRAP1, FRAP2, RAFT1, RAPT1, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1

    Background: Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1.

    ID gène
    2475
    UniProt
    P42345
    Pathways
    Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, L'effet Warburg
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