LATS2 anticorps (pSer83)

N° du produit ABIN753568

Détail du produit anti-LATS2 anticorps

Antigène: LATS2 (LATS, Large Tumor Suppressor, Homolog 2 (Drosophila) (LATS2))

Épitope: pSer83

Reactivité: Souris, Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LATS2 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This phosphorylation site is homologous to that of Ser82 in Mouse and Ser83 Rat.

Réactivité croisée: Humain, Souris, Rat

Homologie: Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human LATS2 around the phosphorylation site of Ser83

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-LATS2 (ABIN753568)

Zhang, Wang, Xie, Chen, Sinkemani, Yu, Wang, Mao, Wu: "Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence." dans: Oncotarget, Vol. 9, Issue 2, pp. 2175-2192, (2018) (PubMed).

Détails sur LATS2

Antigène: LATS2 (LATS, Large Tumor Suppressor, Homolog 2 (Drosophila) (LATS2))

Autre désignation: LATS2 (LATS2 Produits)

Synonymes: anticorps 4932411G09Rik, anticorps AV277261, anticorps AW228608, anticorps KPM, anticorps lats2, anticorps large tumor suppressor kinase 2, anticorps large tumor suppressor 2, anticorps LATS2, anticorps Lats2, anticorps lats2

Sujet:

Synonyms: KPM, Serine/threonine-protein kinase LATS2, Kinase phosphorylated during mitosis protein, Large tumor suppressor homolog 2, Serine/threonine-protein kinase kpm, Warts-like kinase, LATS2

Background: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.

ID gène: 26524

UniProt: Q9NRM7