The antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.
Purification
Affinity purified
Immunogène
Peptide sequence around phosphorylation site of pSer149 (F-R-S (p) -L-P) derived from Mouse Cdc25B. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.