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Keratin 7/17 anticorps

KRT7/17 Reactivité: Humain, Boeuf (Vache), Porc WB, IP, ICC, IHC (p) Hôte: Souris Monoclonal C-46 unconjugated
N° du produit ABIN94296
  • Antigène Voir toutes Keratin 7/17 (KRT7/17) Anticorps
    Keratin 7/17 (KRT7/17)
    Reactivité
    Humain, Boeuf (Vache), Porc
    Hôte
    • 4
    Souris
    Clonalité
    • 4
    Monoclonal
    Conjugué
    • 4
    Cet anticorp Keratin 7/17 est non-conjugé
    Application
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificité
    The antibody C-46 reacts with cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.
    Aucune reactivité croisée
    Souris, Lapin, Rat
    Réactivité croisée (Details)
    Porcine, Human, Bovine
    Purification
    Purified by protein-A affinity chromatography.
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
    Clone
    C-46
    Isotype
    IgG1
  • Indications d'application
    Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, pretreatment: 0,1 % pepsin, 30 min, 37 °C, incubation 4 °C overnight, chromogen DAB, positive tissue: pancreas.
    Western blotting: Recommended dilution: 1-2 μg/mL, positive control: HeLa cells.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Vojt?sek, Stasková, Nenutil, Bártková, Kovarík, Rejthar, Bártek: "A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis." dans: Neoplasma, Vol. 37, Issue 3, pp. 333-42, (1990) (PubMed).

  • Antigène
    Keratin 7/17 (KRT7/17)
    Autre désignation
    Cytokeratin 7+17 (KRT7/17 Produits)
    Synonymes
    anticorps KRT7, anticorps CK7, anticorps keratin 7, anticorps KRT7
    Sujet
    Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K7, K17, CK7, CD17, CK17, CYK7, CYK17, KRT7, KRT17, SCL, K2C7
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