Souris anti-Boeuf (Vache) Ig Light Chains Anticorps

N° du produit ABIN94401

Détail du produit

Antigène: Ig Light Chains

Reactivité: Boeuf (Vache), Mouton

Hôte: Souris

Clonalité: Monoclonal

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Specificité: The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.

Réactivité croisée (Details): Bovine, Sheep

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Fraction of Bovine immunoglobulin.

Clone: IVA285-1

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Recommended dilution: 0,5-1 μg/mL, overnight at 4 °C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12 % separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Référence

Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." dans: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, (2006) (PubMed).

Détail du antigène

Antigène: Ig Light Chains

Sujet: Immunoglobulin light chains