Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)
Specificité
The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
Aucune reactivité croisée
Souris
Réactivité croisée (Details)
Human
Purification
Purified by protein-A affinity chromatography.
Pureté
> 95 % (by SDS-PAGE)
Immunogène
Recombinant intracellular domain (aa 29-186) of human TRIM.
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze.
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
Kirchgessner, Dietrich, Scherer, Isomäki, Korinek, Hilgert, Bruyns, Leo, Cope, Schraven: "The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain." dans: The Journal of experimental medicine, Vol. 193, Issue 11, pp. 1269-84, (2001) (PubMed).
Antigène
TRIM (TRAT1)
(T Cell Receptor Associated Transmembrane Adaptor 1 (TRAT1))
T cell receptor associated transmembrane adaptor 1,TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.,TRAT, TCRIM