STAT1 anticorps (pTyr701)

N° du produit ABIN956953

Cet anticorps Lapin Polyclonal détecte spécifiquement STAT1 dans WB. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour STAT1 anticorps (pTyr701) (ABIN956953)

Antigène: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp STAT1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-STAT1 anticorps

Épitope: AA 694-705, pTyr701

Specificité: Recognizes phosphorylated STAT1, Mr 92kD. Species cross-reactivity: Human, mouse, rat and chicken.

Purification: Immunoaffinity purified

Immunogène: KLH-conjugated synthetic peptide (C-DDPKRTG[pY]IKTE) corresponding to amino acids 694-705 of mouse STAT1, containing a N-terminal cysteine.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: WB (1:500 - 1:2500)

Usage: Suitable for use in Western Blot. Western Blot: 1:500-1:2500. A 1:500-1:2500 dilution detects phosphorylated STAT1 (92kD) in RIPA lysates from CNTF-treated SK-N-MC cells. 20 μg of non-treated or CNTF-treated SK-N-MC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-STAT1 (Y107) (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Western Blot Protocol. 1. Perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on an appropriately treated cell lysate sample (cell lysis buffer: 50 mM Tris-HCl, pH 7.4, 1 % NP-40, 0.25 % sodium deoxycholate, 150 mM NaCl, 1 mM EGTA, 1 mM PMSF, 1 μg/mL each aprotinin, leupeptin, pepstatin, 1 mM Na3VO4, 1 mM NaF) and transfer the proteins to nitrocellulose. Wash the blotted nitrocellulose twice with water. 2. Block the blotted nitrocellulose in freshly prepared TBS containing 5 % nonfat dry milk and 0.05 % Tween-20 (TBST-MLK) for 30-60 minutes at room temperature with constant agitation. 3. Incubate the nitrocellulose with a 1:500-1:2500 dilution of anti-phospho-STAT1 (Y701), diluted in freshly prepared TBST-MLK overnight with agitation at 4?C. 4. Wash the nitrocellulose twice with water. 5. Incubate the nitrocellulose in the secondary reagent of choice (a goat anti-rabbit HRP conjugated IgG, 1:5000 to dilution was used) in TBST-MLK for 1.5 hours at room temperature with agitation. 6. Wash the nitrocellulose with water twice. 7. Wash the nitrocellulose in TBS-0.05 % Tween 20 for 3-5 minutes. 8. Rinse the nitrocellulose in 4-5 changes of water. 9. Use detection method of choice (enhanced chemiluminescence was used).

Not recommended for: IHC-P

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 5 mg/mL BSA, 0.1 % sodium azide, 50 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: +4°C or -20°C, Avoid repeated freezing and thawing.

Détails sur STAT1

Antigène: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Autre désignation: STAT1

Sujet: Name/Gene ID: STAT1

Synonyms: STAT1, ISGF-3, STAT91, CANDF7

ID gène: 6772

UniProt: P42224

Pathways: Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events