VEGF anticorps (HRP)

N° du produit ABIN964773

Détail du produit anti-VEGF anticorps (HRP)

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VEGF est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA

Specificité: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Attributs du produit: VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K -> N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.

Purification: purified

Immunogène: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
Immunogen Type: RecombinantProtein

Isotype: IgG

Information d'application

Indications d'application: This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kDa in size corresponding to monomeric human VEGF-165 protein by western blotting in the appropriate cell lysate or extract.

Commentaires:

Gene Name: VEGFA

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Conseil sur la manipulation: Do NOT add Sodium Azide!

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Références pour anticorps anti-VEGF (HRP) (ABIN964773)

Sreevalsan, Safe: "The cannabinoid WIN 55,212-2 decreases specificity protein transcription factors and the oncogenic cap protein eIF4E in colon cancer cells." dans: Molecular cancer therapeutics, Vol. 12, Issue 11, pp. 2483-93, (2014) (PubMed).

Détails sur VEGF

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Autre désignation: VEGF (VEGF Produits)

Synonymes: anticorps MVCD1, anticorps VEGF, anticorps VPF, anticorps Vegf, anticorps Vegf120, anticorps Vegf164, anticorps Vegf188, anticorps Vpf, anticorps VEGF-A, anticorps VEGF164, anticorps eVEGF120, anticorps eVEGF164, anticorps vegf-a, anticorps vegf, anticorps vascular endothelial growth factor A, anticorps vascular endothelial growth factor A L homeolog, anticorps vascular endothelial growth factor, anticorps vascular endothelial growth factor precursor, anticorps VEGFA, anticorps Vegfa, anticorps vegfa.L, anticorps VEGF, anticorps vegf

Sujet: VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K → N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.
Synonyms: Vascular endothelial growth factor A; VEGF-A; VEGF-165, VEGF165, VEGF isoform L, Vascular permeability factor; VPF

ID gène: 7422

NCBI Accession: NP_001165097