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NEU1 anticorps (Internal Region)

NEU1 Reactivité: Humain WB, ELISA, IP Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN964880
  • Antigène Voir toutes NEU1 Anticorps
    NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))
    Épitope
    • 8
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivité
    • 49
    • 11
    • 11
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 41
    • 11
    Lapin
    Clonalité
    • 43
    • 9
    Polyclonal
    Conjugué
    • 27
    • 8
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp NEU1 est non-conjugé
    Application
    • 40
    • 20
    • 16
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Specificité
    This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.  This antibody reacts with human Neu2.  Based on sequence we expect this antibody to react with neuraminidase from other sources, although specific reactivity has not been confirmed.  Cross-reactivity against Neu1 has not yet been established.  Neuraminidases are highly conserved in mammals and therefore cross reactivity is expected with mouse and rat Neu2.
    Attributs du produit
    Neuraminidases or sialidases are exoglycosidases that catalyze the cleavage of alpha-glycosidically linked terminal N-acetyl neuraminic acid from sialylated glycoconjugates. They are widely spread in nature, occurring in viruses, bacteria, fungi, protozoa, birds and mammals. Together, the neuraminidases form a family of hydrolases that share a conserved active site and similar sequence motifs. Three types of neuraminidase are found in mammals and are defined as lysosomal, plasma membrane and cytosolic on the basis of their biochemical properties and subcellular distribution. Lysosomal N-acetyl-alpha-neuraminidase (NEU1) has significant primary structure characteristics of other mammalian and microbial sialidases with similar substrate specificity. However, unlike other members of this family, lysosomal neuraminidase requires the carboxypeptidase protective protein/cathepsin A (PPCA) for intracellular transport and lysosomal activation. The enzyme is only catalytically active when it is bound to PPCA and is a component of a high molecular weight, multi-protein complex containing PPCA, ß-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase. Using a hamster Sial3 probe, Monti et al. (1999) identified the gene encoding sialidase-2, which they designated NEU2, from a human genomic library. The 2 putative exons of NEU2 encode a deduced 380-amino acid protein with a calculated molecular mass of 42.23 kD. The NEU2 protein has significant homology with the mammalian, viral, and bacterial sialidases. It shares over 72% similarity with the hamster and rat cytosolic sialidases and over 42% similarity with human NEU1. NEU2 contains a potential N-linked glycosylation site, 2 aspartic acid block consensus sequences, and an N-terminal F/YRIP sequence motif which is part of the active site of other sialidase enzymes. Monti et al. hypothesized that NEU2 has a cytosolic localization because it does not contain a cleavage site, transmembrane domain, or targeting motifs.
    Stérilité
    Sterile filtered
    Immunogène
    This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 110-124 of Human Neu2.
    Immunogen Type: Peptide
    Isotype
    IgG
    Top Product
    Discover our top product NEU1 Anticorps primaire
  • Indications d'application
    This antibody is suitable for western blotting, immunocytochemistry, immunoprecipitation, transfected cell culture, primary cell culture, and ELISA. A single band of the expected apparent molecular weight (43 kDa) was observed at a 1:500 dilution incubated for 1 h at room temperature. A second lower molecular weight band may represent a truncated form of this protein. Neuraminidase is not very abundant in most tissues and its detection using this antibody may require further optimization. Researchers should determine optimal titers for other applications.
    Commentaires

    Gene Name: NEU2

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.9 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Date de péremption
    12 months
  • Antigène
    NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))
    Autre désignation
    Neuraminidase (NEU1 Produits)
    Synonymes
    anticorps NANH, anticorps NEU, anticorps SIAL1, anticorps MGC81958, anticorps AA407268, anticorps AA407316, anticorps Aglp, anticorps Apl, anticorps Bat-7, anticorps Bat7, anticorps G9, anticorps Map-2, anticorps Neu, anticorps Neu-1, anticorps neuraminidase 1, anticorps neuraminidase 1 (lysosomal sialidase) L homeolog, anticorps NEU1, anticorps neu1.L, anticorps neu1, anticorps Neu1
    Classe de substances
    Influenza Protein
    Sujet
    Neuraminidases or sialidases are exoglycosidases that catalyze the cleavage of α-glycosidically linked terminal N-acetyl neuraminic acid from sialylated glycoconjugates. They are widely spread in nature, occurring in viruses, bacteria, fungi, protozoa, birds and mammals. Together, the neuraminidases form a family of hydrolases that share a conserved active site and similar sequence motifs. Three types of neuraminidase are found in mammals and are defined as lysosomal, plasma membrane and cytosolic on the basis of their biochemical properties and subcellular distribution. Lysosomal N-acetyl-α-neuraminidase (NEU1) has significant primary structure characteristics of other mammalian and microbial sialidases with similar substrate specificity. However, unlike other members of this family, lysosomal neuraminidase requires the carboxypeptidase protective protein/cathepsin A (PPCA) for intracellular transport and lysosomal activation. The enzyme is only catalytically active when it is bound to PPCA and is a component of a high molecular weight, multi-protein complex containing PPCA, ß-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase. Using a hamster Sial3 probe, Monti et al. (1999) identified the gene encoding sialidase-2, which they designated NEU2, from a human genomic library. The 2 putative exons of NEU2 encode a deduced 380-amino acid protein with a calculated molecular mass of 42.23 kD. The NEU2 protein has significant homology with the mammalian, viral, and bacterial sialidases. It shares over 72% similarity with the hamster and rat cytosolic sialidases and over 42% similarity with human NEU1. NEU2 contains a potential N-linked glycosylation site, 2 aspartic acid block consensus sequences, and an N-terminal F/YRIP sequence motif which is part of the active site of other sialidase enzymes. Monti et al. hypothesized that NEU2 has a cytosolic localization because it does not contain a cleavage site, transmembrane domain, or targeting motifs.
    Synonyms: Acetylneuraminyl hydrolase antibody, G9 sialidase antibody, Lysosomal sialidase antibody, N acetyl alpha neuraminidase 1 antibody, NANH antibody, Neu 1 antibody
    ID gène
    4759, 222352169
    UniProt
    Q9Y3R4
    Pathways
    SARS-CoV-2 Protein Interactome
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