Chèvre anti-Lapin IgG (Heavy & Light Chain) Anticorps (Atto 647N)

Détails pour le produit réf. ABIN964991
Antigène
Épitope
Heavy & Light Chain
840
233
200
38
13
8
Reactivité
Lapin
222
197
165
143
124
76
75
57
53
53
44
41
40
35
23
14
13
7
7
7
3
2
Hôte
Chèvre
575
499
185
44
36
32
7
7
1
Clonalité
Polyclonal
Conjugué
Atto 647N
181
161
138
131
129
127
46
35
32
29
28
24
16
11
9
9
9
9
7
6
5
5
5
5
5
5
5
5
4
4
4
4
2
2
2
2
1
1
1
1
Application
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Options
Immunogène

Immunogen: Rabbit IgG whole molecule

Isotype IgG
Specificité Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Réactivité croisée (Details) Pre-Adsorbed
Attributs du produit Anti-Rabbit IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification Rabbit IgG (H&L) Antibody ATTO 647N was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.  This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
Labeling Ratio 2.5
Antigène
Sujet

Synonyms: Goat anti-Rabbit IgG Antibody ATTO647N Conjugation, Goat anti-Rabbit IgG ATTO 647N Conjugated Antibody

Background: Anti-Rabbit IgG (H&L) ATTO 647N Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Indications d'application

Application Note: Anti-Rabbit IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Commentaires

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid cycles of freezing and thawing.
Dilute only prior to immediate use
Product is photosensitive and should be protected from light.
Stock RT,4 °C,-20 °C
Stockage commentaire Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
Date de péremption 12 months
Images (Fournisseur)
 image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) (ABIN964991) Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N)
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) (ABIN964991) Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs,...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) (ABIN964991) ATTO 647N conjugated anti rabbit antibody was used to detect anti-Beta Actin antibody...
Produit citée dans: Truckenbrodt, Viplav, Jähne, Vogts, Denker, Wildhagen, Fornasiero, Rizzoli: "Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission." dans: The EMBO journal, Vol. 37, Issue 15, 2019 (PubMed).

Hruska, Henderson, Le Marchand, Jafri, Dalva: "Synaptic nanomodules underlie the organization and plasticity of spine synapses." dans: Nature neuroscience, Vol. 21, Issue 5, pp. 671-682, 2019 (PubMed).

Gomes de Castro, Höbartner, Opazo: "Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy." dans: PLoS ONE, Vol. 12, Issue 2, pp. e0173050, 2017 (PubMed).

Saka, Vogts, Kröhnert, Hillion, Rizzoli, Wessels: "Correlated optical and isotopic nanoscopy." dans: Nature communications, Vol. 5, pp. 3664, 2015 (PubMed).