Chèvre anti-Rat (Rattus) IgG (Heavy & Light Chain) Anticorps (Atto 550)

Détails pour le produit réf. ABIN965003
Antigène
Épitope
Heavy & Light Chain
840
233
200
38
13
8
Reactivité
Rat (Rattus)
222
198
165
142
124
76
75
57
53
53
44
41
40
35
23
14
13
7
7
7
3
2
Hôte
Chèvre
575
499
185
44
36
32
7
7
1
Clonalité
Polyclonal
Conjugué
Atto 550
181
161
138
131
129
127
46
35
32
29
28
24
16
11
9
9
9
9
7
6
5
5
5
5
5
5
5
5
4
4
4
4
2
2
2
2
1
1
1
1
Application
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Options
Immunogène

Immunogen: Rat IgG whole molecule

Isotype IgG
Specificité Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.
Réactivité croisée (Details) Pre-Adsorbed
Attributs du produit Anti-Rat IgG (H&L) conjugated to ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification Rat IgG (H&L) Antibody ATTO 550 was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.  This antibody will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins.
Labeling Ratio 2.5
Antigène
Sujet

Synonyms: Goat anti-Rat IgG ATTO550 Conjugated Antibody, Goat anti-Rat IgG Antibody ATTO 550 Conjugation

Background: Anti-Rat IgG (H&L) conjugated to ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Indications d'application

Application Note: The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Commentaires

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light.
Stock RT,4 °C,-20 °C
Stockage commentaire Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
Date de péremption 12 months
Images (Fournisseur)
 image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 550) (ABIN965003) Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 550)
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 550) (ABIN965003) Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb...
 image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 550) (ABIN965003) Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 550) (Image 3)