Chèvre anti-Rat (Rattus) IgG (Heavy & Light Chain) Anticorps (Atto 550)

Détails pour le produit réf. ABIN965003, Fournisseur: Connectez-vous pour afficher
Antigène
Épitope
Heavy & Light Chain
6508
2040
1893
1189
814
645
311
269
33
30
30
15
12
12
12
12
11
7
3
2
1
1
1
1
1
1
1
Reactivité
Rat (Rattus)
3157
3006
2460
1723
1559
805
759
503
483
429
411
409
335
291
276
172
159
119
54
30
30
26
15
12
12
8
7
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Hôte
Chèvre
7403
5360
2056
1078
543
357
89
73
55
35
23
15
8
5
1
1
1
Clonalité
Polyclonal
Conjugué
Atto 550
2298
1889
1829
1326
792
657
536
340
235
146
145
140
140
117
112
112
102
97
94
93
92
91
89
84
81
79
78
75
73
72
68
68
65
61
55
54
54
54
54
53
53
53
53
53
53
52
48
43
41
36
32
31
23
23
22
20
19
18
17
16
15
15
15
14
14
14
12
12
12
12
11
11
11
10
10
10
10
9
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Options
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N° du produit (Fournisseur)
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Immunogène

Immunogen: Rat IgG whole molecule

Isotype IgG
Specificité Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.
Réactivité croisée (Details) Pre-Adsorbed
Attributs du produit Anti-Rat IgG (H&L) conjugated to ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification Rat IgG (H&L) Antibody ATTO 550 was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.  This antibody will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins.
Labeling Ratio 2.5
Antigène
Sujet

Synonyms: Goat anti-Rat IgG ATTO550 Conjugated Antibody, Goat anti-Rat IgG Antibody ATTO 550 Conjugation

Background: Anti-Rat IgG (H&L) conjugated to ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Indications d'application

Application Note: The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Commentaires

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light.
Stock RT,4 °C,-20 °C
Stockage commentaire Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
Date de péremption 12 months
Images (Fournisseur)
 image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 550) (ABIN965003) Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 550)
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 550) (ABIN965003) Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb...
 image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 550) (ABIN965003) Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 550) (Image 3)