Chèvre anti-Hamster arménien IgG (Heavy & Light Chain) Anticorps (FITC) - Preadsorbed

Détails pour le produit réf. ABIN965013
Antigène
Épitope
Heavy & Light Chain
845
233
200
38
13
8
Reactivité
Hamster arménien, Hamsters dorés syriens
222
200
165
143
124
76
75
57
53
53
44
40
40
35
23
13
13
13
7
7
6
3
2
Hôte
Chèvre
575
499
185
43
38
36
7
7
3
1
Clonalité
Polyclonal
Conjugué
FITC
180
163
138
133
129
127
46
35
32
28
28
24
16
11
9
9
9
9
7
6
5
5
5
5
5
5
5
5
5
4
4
4
2
2
2
2
1
1
1
1
1
1
1
Application
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Options
Immunogène

Immunogen: Armenian and Golden Syrian Hamster IgG, whole molecule

Isotype IgG
Specificité Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Armenian/Golden Syrian Hamster IgG and Armenian/Golden Syrian Hamster Serum.
Attributs du produit This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification Preadsorption: Solid phase absorption
Stérilité Sterile filtered
Labeling Ratio 4.1
Antigène
Sujet

Synonyms: goat anti-Golden Syrian & Armenian Hamster IgG fluorescein conjugated antibody, goat anti-Hamster IgG FITC conjugated antibody

Background: Anti-Golden Syrian & Armenian Hamster IgG Fluorescein Antibody generated in goat detects Golden Syrian & Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Indications d'application

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000-1:50,000

Flow Cytometry Dilution: User Optimized

IF Microscopy Dilution: 1:500-1:2,500

Commentaires

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
Stock RT,4 °C,-20 °C
Stockage commentaire Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
Date de péremption 12 months