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Lapin anti-Hamsters dorés syriens IgG (Heavy & Light Chain) Anticorps (TRITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG TRITC F(ab')2 fragment
N° du produit ABIN965064
  • Antigène Tous les produits IgG
    IgG
    Fragment
    F(ab')2 fragment
    Épitope
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivité
    • 695
    • 672
    • 561
    • 458
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 96
    • 95
    • 92
    • 62
    • 47
    • 38
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Hamsters dorés syriens
    Hôte
    • 1730
    • 1473
    • 539
    • 206
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Lapin
    Clonalité
    • 3360
    • 167
    • 1
    Polyclonal
    Conjugué
    • 557
    • 409
    • 406
    • 368
    • 298
    • 275
    • 228
    • 121
    • 99
    • 67
    • 66
    • 62
    • 62
    • 52
    • 48
    • 47
    • 45
    • 44
    • 44
    • 41
    • 39
    • 34
    • 33
    • 33
    • 31
    • 31
    • 30
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    TRITC
    Application
    • 1840
    • 1799
    • 1792
    • 1501
    • 962
    • 933
    • 912
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 52
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Specificité
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum.
    Attributs du produit
    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Golden Syrian Hamster IgG whole molecule

    Isotype
    IgG
    Labeling Ratio
    2.0
  • Indications d'application

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Commentaires

    Post Translational Modification: Phosphorylation

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid cycles of freezing and thawing.
    Product is photosensitive and should be protected from light.
    Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
    Date de péremption
    12 months
  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: Rabbit F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Antibody Rhodamine Conjugation, Rabbit F(ab')2 Anti-Hamster IgG Rhodamine Conjugated Antibody, Rabbit Fab2 Anti-Hamster IgG TRITC Conjugated Antibody

    Background: F(ab')2 Anti-Golden Syrian Hamster IgG Rhodamine Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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