Lapin anti-Cheval IgG (Heavy & Light Chain) Anticorps (Biotin) - Preadsorbed

N° du produit ABIN965074

Détail du produit

Antigène: IgG

Fragment: F(ab')2 fragment

Épitope: Heavy & Light Chain

Reactivité: Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Horse IgG and Horse Serum.

Purification: Preadsorption: Solid phase absorption

Stérilité: Sterile filtered

Immunogène:

Immunogen: Horse IgG whole molecule

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:2,500

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

ELISA Dilution: 1:300,000

Western Blot Dilution: 1:10,000 - 1:25,000

Commentaires:

Post Translational Modification: Phosphorylation

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid cycles of freezing and thawing.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Rabbit F(ab')2 Anti-Horse IgG Antibody Biotin Conjugation, Rabbit F(ab')2 Anti-Horse IgG Biotin Conjugated Antibody, Rabbit Fab2 Anti-Horse IgG Biotin Conjugated Antibody

Background: F(ab')2 Anti-Horse IgG Biotin Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.