p105 Proliferation-Associatedantigen (Nuclear) anticorps

Détails pour le produit réf. ABIN966725, Fournisseur: Connectez-vous pour afficher
Antigène
Épitope
Nuclear
Reactivité
Humain
Hôte
Souris
Clonalité (Clone)
Monoclonal ()
Application
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)
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Immunogène Activated peripheral blood nuclei.
Clone B301
Isotype IgM
Specificité The p105 protein, found in all human cells in a dimeric form (105 kD) and monomeric form (41 kD), is a proliferation-associated nuclear antigen that is absent in non-cycling cells in the G0 phase. This protein is important in the synthesis and transport of RNA in the cell regulation cycle. During G2 and mitosis, this protein is increased possibly due to the unmasking of nucleolar antigens, resulting in large accumulations of this protein in the mitotic cytoplasm.
Purification Concentrated.
Antigène
Poids moléculaire Dimeric form 105 kDa, Monomeric form 41 kDa
ID gène 4790
Indications d'application Immunohistochemistry: 1:50-1:100. Formalin-fixed paraffin-embedded (FFPE) tissue sections require pepsin or trypsin digestion.
Western blotting: 1:100-1:200 .
Staining Procedure: This antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. FFPE tissue sections require pepsin or trypsin digestion. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.
Commentaires

Cellular Localization: Nuclear.
Recommended Positive Control: Human tonsil or lymph node.

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Background publications Clevenger, Bauer, Epstein: "A method for simultaneous nuclear immunofluorescence and DNA content quantitation using monoclonal antibodies and flow cytometry." dans: Cytometry, Vol. 6, Issue 3, pp. 208-14, 1985 (PubMed).

Clevenger, Epstein: "Identification of a nuclear protein component of interchromatin granules using a monoclonal antibody and immunogold electron microscopy." dans: Experimental cell research, Vol. 151, Issue 1, pp. 194-207, 1984 (PubMed).

Clevenger, Epstein: "Use of immunogold electron microscopy and monoclonal antibodies in the identification of nuclear substructures." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 32, Issue 7, pp. 757-65, 1984 (PubMed).

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