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p34 Cdc-2protein (Nuclear) anticorps

Reactivité: Humain IHC, WB Hôte: Souris Monoclonal B322 (POH-1) unconjugated
N° du produit ABIN966737
  • Antigène
    p34 Cdc-2protein
    Épitope
    Nuclear
    Reactivité
    Humain
    Hôte
    Souris
    Clonalité
    Monoclonal
    Application
    Immunohistochemistry (IHC), Western Blotting (WB)
    Specificité
    This antibody reacts with a 34 kD cyclic dependent serine/threonine kinase protein. P34 cdc2 and cyclin B1 are catalytic subunits of the maturation promoting factor (MPF). This antigen is activated with cyclins through dephosphrylation of tyrosine residue, initiates the entry of cells into mitosis, and is followed by the breakdown of the nuclear envelope. Expression of this antigen reflects the proliferating potential of positively staining tissue.
    Purification
    Concentrated.
    Immunogène
    The recombinant human p34.cdc2 fusion protein.
    Clone
    B322 (POH-1)
    Isotype
    IgG2a
  • Indications d'application
    Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/ml.
    Buffer
    20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
  • Antigène
    p34 Cdc-2protein
    ID gène
    983
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