p34 Cdc-2protein (Nuclear) anticorps

Détails pour le produit réf. ABIN966737, Fournisseur: Connectez-vous pour afficher
Antigène
Épitope
Nuclear
Reactivité
Humain
Hôte
Souris
Clonalité (Clone)
Monoclonal ()
Application
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogène The recombinant human p34.cdc2 fusion protein.
Clone B322 (POH-1)
Isotype IgG2a
Specificité This antibody reacts with a 34 kD cyclic dependent serine/threonine kinase protein. P34 cdc2 and cyclin B1 are catalytic subunits of the maturation promoting factor (MPF). This antigen is activated with cyclins through dephosphrylation of tyrosine residue, initiates the entry of cells into mitosis, and is followed by the breakdown of the nuclear envelope. Expression of this antigen reflects the proliferating potential of positively staining tissue.
Purification Concentrated.
Antigène
ID gène 983
Indications d'application Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Background publications Pines, Hunter: "Cyclin-dependent kinases: a new cell cycle motif?" dans: Trends in cell biology, Vol. 1, Issue 5, pp. 117-21, 2004 (PubMed).

Lukás, Draetta, Bartek: "Distinct forms of human CDC2 identified by novel monoclonal antibodies." dans: European journal of biochemistry / FEBS, Vol. 207, Issue 1, pp. 169-76, 1992 (PubMed).

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