pan Keratin (panKRT) anticorps

Détails pour le produit réf. ABIN966802, Fournisseur: Connectez-vous pour afficher
Antigène
  • xk70a
  • keratin
  • keratin S homeolog
  • LOC431321
  • krt.S
Reactivité
Humain
166
86
79
25
24
13
11
11
11
10
9
7
6
3
2
2
2
2
Hôte
Souris
106
70
2
Clonalité (Clone)
Monoclonal ()
Conjugué
Inconjugué
8
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
81
72
66
45
40
37
24
21
19
15
5
2
2
1
1
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Immunogène Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.
Clone B311-1
Isotype IgG1 kappa
Specificité This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond tocytokeratins 4,5,6,8,10,13 and 18.
Purification Concentrated.
Autre désignation Pan Cytokeratin, Pan CK-II
Poids moléculaire 45, 52.5 ,54, 56, 56.5, 58, & 59 kDa
Indications d'application IHC (FFPE): 1:50-1:100 (Requires high temp. antigen demaking, 10 mM Citrate pH 6.0 boiling tissue for 10-20 mins. followed by cooling at RT for 10-20 mins or protease digestion).
Western blotting: 1:100-1:400
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC, with High temperature antigenic unmasking technique predigestion is recommended with this antibody when using paraffin-embedded tissue sections.
Commentaires

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Skin, Colonic Carcinoma

Restrictions For Research Use only
Format Liquid
Concentration 0.35 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Background publications Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." dans: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Kasper: "Heterogeneity in the immunolocalization of cytokeratin specific monoclonal antibodies in the rat eye: evaluation of unusual epithelial tissue entities." dans: Histochemistry, Vol. 95, Issue 6, pp. 613-20, 1991 (PubMed).

Bártková, Bártek, Lukás, Vojt?sek, Stasková, Bursová, Pavlovská, Rejthar, Kovarík: "Effects of tissue fixation conditions and protease pretreatment on immunohistochemical performance of a large series of new anti-keratin monoclonal antibodies: value in oncopathology." dans: Neoplasma, Vol. 38, Issue 4, pp. 439-46, 1991 (PubMed).

Avez-vous cherché autre chose?