pan Keratin (panKRT) anticorps

Détails pour le produit réf. ABIN966802, Fournisseur: Connectez-vous pour afficher
Antigène
  • xk70a
  • keratin
  • keratin S homeolog
  • LOC431321
  • krt.S
Reactivité
Humain
304
198
170
112
74
67
57
55
54
52
50
49
25
11
2
2
2
2
2
Hôte
Souris
217
95
4
Clonalité (Clone)
Monoclonal ()
Conjugué
Inconjugué
10
10
9
8
8
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
3
3
3
3
3
3
3
3
3
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
179
178
168
100
87
65
40
31
26
22
15
5
2
1
1
Options
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N° du produit (Fournisseur)
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Immunogène Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.
Clone B311-1
Isotype IgG1 kappa
Specificité This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond tocytokeratins 4,5,6,8,10,13 and 18.
Purification Concentrated.
Autre désignation Pan Cytokeratin, Pan CK-II
Poids moléculaire 45, 52.5 ,54, 56, 56.5, 58, & 59 kDa
Indications d'application IHC (FFPE): 1:50-1:100 (Requires high temp. antigen demaking, 10 mM Citrate pH 6.0 boiling tissue for 10-20 mins. followed by cooling at RT for 10-20 mins or protease digestion).
Western blotting: 1:100-1:400
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC, with High temperature antigenic unmasking technique predigestion is recommended with this antibody when using paraffin-embedded tissue sections.
Commentaires

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Skin, Colonic Carcinoma

Restrictions For Research Use only
Format Liquid
Concentration 0.35 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Background publications Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." dans: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Kasper: "Heterogeneity in the immunolocalization of cytokeratin specific monoclonal antibodies in the rat eye: evaluation of unusual epithelial tissue entities." dans: Histochemistry, Vol. 95, Issue 6, pp. 613-20, 1991 (PubMed).

Bártková, Bártek, Lukás, Vojt?sek, Stasková, Bursová, Pavlovská, Rejthar, Kovarík: "Effects of tissue fixation conditions and protease pretreatment on immunohistochemical performance of a large series of new anti-keratin monoclonal antibodies: value in oncopathology." dans: Neoplasma, Vol. 38, Issue 4, pp. 439-46, 1991 (PubMed).

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