IKBKG anticorps

N° du produit ABIN967646

Détail du produit anti-IKBKG anticorps

Antigène: IKBKG (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IKBKG est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Marque: BD Pharmingen™

Réactivité croisée: Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Recombinant Human IKKgamma

Clone: C73

Isotype: IgG1 kappa

Information d'application

Indications d'application: Applications include western blot analysis and immunoprecipitation. In immunoprecipitation experiments, it is reported that the C73-764 antibody brings down the IKK complex (IKKalpha, IKKbeta, and IKKgamma). The immunoprecipitation application has not been evaluated. NIH/3T3 cells (ATCC CRL 1658) are recommended as a western blot positive control.

Commentaires:

Related Products: ABIN968538, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-IKBKG (ABIN967646)

Chu, Ostertag, Li, Chang, Chen, Hu, Williams, Perrault, Karin: "JNK2 and IKKbeta are required for activating the innate response to viral infection." dans: Immunity, Vol. 11, Issue 6, pp. 721-31, (2000) (PubMed).

Li, Chu, Hu, Delhase, Deerinck, Ellisman, Johnson, Karin: "The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis." dans: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1839-45, (1999) (PubMed).

Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, (1998) (PubMed).

Nakano, Shindo, Sakon, Nishinaka, Mihara, Yagita, Okumura: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, (1998) (PubMed).

Rothwarf, Zandi, Natoli, Karin: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." dans: Nature, Vol. 395, Issue 6699, pp. 297-300, (1998) (PubMed).

DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." dans: Nature, Vol. 388, Issue 6642, pp. 548-54, (1997) (PubMed).

Mercurio, Zhu, Murray, Shevchenko, Bennett, Li, Young, Barbosa, Mann, Manning, Rao: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." dans: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, (1997) (PubMed).

Détails sur IKBKG

Antigène: IKBKG (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))

Autre désignation: IKK gamma (IKBKG Produits)

Synonymes: anticorps AMCBX1, anticorps FIP-3, anticorps FIP3, anticorps Fip3p, anticorps IKK-gamma, anticorps IP, anticorps IP1, anticorps IP2, anticorps IPD2, anticorps NEMO, anticorps ZC2HC9, anticorps 1110037D23Rik, anticorps AI848108, anticorps AI851264, anticorps AW124339, anticorps IKK[g], anticorps Nemo, anticorps nemo, anticorps fj33c07, anticorps wu:fj33c07, anticorps zgc:113492, anticorps MGC97885, anticorps IKBKG, anticorps IKBKG_1, anticorps IKBKG_2, anticorps CG16910, anticorps DmIKK-gamma, anticorps DmIKKgamma, anticorps Dmel\\CG16910, anticorps Dmikkgamma, anticorps IKK, anticorps IKK[[gamma]], anticorps IKKg, anticorps IKKgamma, anticorps KEY, anticorps Kenny, anticorps Key, anticorps dIKK, anticorps dIKK-gamma, anticorps dmIKKgamma, anticorps inhibitor of nuclear factor kappa B kinase subunit gamma, anticorps inhibitor of kappaB kinase gamma, anticorps inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma, anticorps inhibitor of nuclear factor kappa B kinase subunit gamma L homeolog, anticorps kenny, anticorps IKBKG, anticorps Ikbkg, anticorps ikbkg, anticorps ikbkg.L, anticorps IKBKG_1, anticorps key

Sujet: The transcription factor NF-kappaB, (nuclear factor kappa-B) is controlled by interaction with an inhibitory subunit, IkappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, IkappaB becomes degraded and NF-kappaB is released to the nucleus. The release of IkappaB from NF-kappaB is a critical step in the activation of NF-kappaB signal pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKalpha and IKKbeta (aka IKK-1 and IKK-2), which phosphorylate critical residues of IkappaB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKalpha, IKKbeta, as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKgamma. IKKalpha and IKKbeta become activated following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK) and MEKK1. IKKgamma interacts preferentially with IKKbeta and is required for the activation of the IKK complex. Thus the IKK kinases play an important role in the activation of NF-kappaB. IKKgamma migrates as a doublet between 50-55 kDa in SDS-PAGE.
Synonyms: NEMO

Poids moléculaire: 50-55 kDa doublet

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins