RASA1 anticorps (AA 381-390)

N° du produit ABIN967706

Détail du produit anti-RASA1 anticorps

Antigène: RASA1 (RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1))

Épitope: AA 381-390

Reactivité: Humain, Rat, Souris, Chien, Poulet, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RASA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Chien, Rat (Rattus), Souris, Poulet, Grenouille

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Ras-GAP aa.381-390

Clone: 13-RAS

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968619, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-RASA1 (ABIN967706)

Cichowski, Santiago, Jardim, Johnson, Jacks: "Dynamic regulation of the Ras pathway via proteolysis of the NF1 tumor suppressor." dans: Genes & development, Vol. 17, Issue 4, pp. 449-54, (2003) (PubMed).

Sato, Ogata, De Luca: "Annexin V inhibits the 12-O-tetradecanoylphorbol-13-acetate-induced activation of Ras/extracellular signal-regulated kinase (ERK) signaling pathway upstream of Shc in MCF-7 cells." dans: Oncogene, Vol. 19, Issue 25, pp. 2904-12, (2000) (PubMed).

Dupont, Blancq: "Formation of complexes involving RasGAP and p190 RhoGAP during morphogenetic events of the gastrulation in xenopus." dans: European journal of biochemistry / FEBS, Vol. 265, Issue 2, pp. 530-8, (1999) (PubMed).

Kaplan, Morrison, Wong, McCormick, Williams: "PDGF beta-receptor stimulates tyrosine phosphorylation of GAP and association of GAP with a signaling complex." dans: Cell, Vol. 61, Issue 1, pp. 125-33, (1990) (PubMed).

Molloy, Bottaro, Fleming, Marshall, Gibbs, Aaronson: "PDGF induction of tyrosine phosphorylation of GTPase activating protein." dans: Nature, Vol. 342, Issue 6250, pp. 711-4, (1990) (PubMed).

Détails sur RASA1

Antigène: RASA1 (RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1))

Autre désignation: Ras-GAP (RASA1 Produits)

Synonymes: anticorps CG9209, anticorps D-RasGAP, anticorps Dmel\\CG9209, anticorps RAS-GAP, anticorps RasGAP, anticorps RasGap, anticorps rasGap, anticorps CM-AVM, anticorps CMAVM, anticorps GAP, anticorps PKWS, anticorps RASA, anticorps RASGAP, anticorps p120GAP, anticorps p120RASGAP, anticorps GAPX, anticorps Rasa, anticorps Gap, anticorps vacuolar peduncle, anticorps RAS p21 protein activator 1, anticorps vap, anticorps RASA1, anticorps Rasa1

Sujet: Ras-GAP, a protein of approximately 120kDa, is thought to serve as a down-regulator of p21ras by stimulating its otherwise weak intrinsic GTPase activity. Both growth factor receptor and oncogene-encoded tyrosine kinases require functional membrane-associated Ras proteins in order to affect mitogenesis. The manner in which p21ras acts as a signal transducer is still a matter of speculation. The Ras-GTPase stimulating activity has been found to reside in the carboxy-terminal region of Ras-GAP, while the amino-terminal region contains two SH2 domains and an intervening SH3 domain. In cells stimulated with EGF or transformed by pp60 [v-src], Ras-GAP becomes phosphorylated on both tyrosine and serine residues and forms distinct complexes with two phosphorylated proteins of 62 and 190kDa. The tyrosine phosphorylation of Ras-GAP may modulate its subcellular localization and activity as a negative regulator of p21ras.
This antibody routinely tested by western blot analysis.

Poids moléculaire: 120 kDa

Pathways: Regulation of Actin Filament Polymerization, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals