Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Casein Kinase II alpha /alpha (AA 1-123) anticorps

Reactivité: Humain, Souris, Rat FACS, WB Hôte: Souris Monoclonal 31-Casein Kinase IIalpha-alpha' unconjugated
N° du produit ABIN968609
  • Antigène
    Casein Kinase II alpha /alpha
    Épitope
    AA 1-123
    Reactivité
    Humain, Souris, Rat
    Hôte
    Souris
    Clonalité
    Monoclonal
    Application
    Flow Cytometry (FACS), Western Blotting (WB)
     Réactivité croisée
    Humain, Souris
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Rat Casein Kinase II alpha aa. 1-123
    Clone
    31-Casein Kinase IIalpha-alpha'
    Isotype
    IgG1
  • Commentaires

    Related Products: ABIN968546, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20°C.
  • Freeman, Brebner, Lynch, Patel, Robertson, Roberts, Vrana: "Changes in rat frontal cortex gene expression following chronic cocaine." dans: Brain research. Molecular brain research, Vol. 104, Issue 1, pp. 11-20, (2002) (PubMed).

    Greer, Wang, Raman, Justement: "CD45 function is regulated by an acidic 19-amino acid insert in domain II that serves as a binding and phosphoacceptor site for casein kinase 2." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 12, pp. 7208-18, (2001) (PubMed).

    Xu, Toselli, Russell, Seldin: "Globozoospermia in mice lacking the casein kinase II alpha' catalytic subunit." dans: Nature genetics, Vol. 23, Issue 1, pp. 118-21, (1999) (PubMed).

    Kikkawa, Mann, Firtel, Hunter: "Molecular cloning of casein kinase II alpha subunit from Dictyostelium discoideum and its expression in the life cycle." dans: Molecular and cellular biology, Vol. 12, Issue 12, pp. 5711-23, (1992) (PubMed).

    Lozeman, Litchfield, Piening, Takio, Walsh, Krebs: "Isolation and characterization of human cDNA clones encoding the alpha and the alpha' subunits of casein kinase II." dans: Biochemistry, Vol. 29, Issue 36, pp. 8436-47, (1991) (PubMed).

  • Antigène
    Casein Kinase II alpha /alpha
    Sujet
    Casein kinase II (CKII, CK2) is a pleiotropic, ubiquitous, and constitutively active Ser/Thr kinase that utilizes both ATP and GTP as phosphoryl donors to catalyze the phosphorylation of numerous protein substrates. It has been implicated in DNA replication, control of metabolism, and regulation of transcription and translation. CKII is a messenger independent kinase due to the fact that its activity is not affected by factors that regulate other protein kinases (e.g. cAMP, Ca2+, diacylglycerol). It is composed of two catalytic (alpha and alpha') subunits and two regulatory (beta) subunits. The beta subunits stabilize the holoenzyme and enhance the activity of the alpha subunits. Although the enzyme exists as a tetramer, the free alpha/alpha' subunits are catalytically active by themselves and are present in cells under some conditions. The alpha and alpha' subunits (85% homologous) are encoded by distinct genes and have been detected in multiple species including human, bovine, and avian. CKII is found primarily in the nucleus in dividing cells, but can be detected in the cytoplasm in quiescent cells. Thus, CKII activity is required for cell cycle progression and other essential signal transduction events.
    Poids moléculaire
    45 kDa
Vous êtes ici:
Support technique