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CREB1 anticorps

CREB1 Reactivité: Humain, Souris, Rat, Singe WB, IHC, ELISA, ICC Hôte: Souris Monoclonal 5G3 unconjugated
N° du produit ABIN969061
  • Antigène Voir toutes CREB1 Anticorps
    CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
    Reactivité
    • 291
    • 213
    • 179
    • 33
    • 24
    • 18
    • 17
    • 14
    • 12
    • 9
    • 9
    • 8
    • 8
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Singe
    Hôte
    • 265
    • 28
    • 1
    • 1
    Souris
    Clonalité
    • 240
    • 55
    Monoclonal
    Conjugué
    • 148
    • 16
    • 14
    • 12
    • 12
    • 11
    • 11
    • 11
    • 11
    • 10
    • 10
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    Cet anticorp CREB1 est non-conjugé
    Application
    • 224
    • 99
    • 98
    • 73
    • 56
    • 56
    • 48
    • 47
    • 20
    • 17
    • 16
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)
    Purification
    purified
    Immunogène
    Purified recombinant fragment of human CREB1 expressed in E. coli.
    Clone
    5G3
    Isotype
    IgG1
  • Indications d'application
    ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000
    Restrictions
    For Research Use only
  • Validation #100101 (Western Blotting)
    'Independent Validation' signe
    by
    Instituto de Parasitología y Biomedicina López-Neyra
    No.
    #100101
    Date
    23.12.2016
    Antigène
    CREB1
    Numéro du lot
    110517
    Application validée
    Western Blotting
    Contrôle positif
    primary mouse microglia
    Contrôle négative
    siRNA knock-down of CREB1 in primary mouse microglia
    Conclusion
    Passed. ABIN969061 specifically recognizes Creb1 in primary mouse microglia lysates.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN969061
    Anticorps secondaire
    anti-mouse HRP-conjugated antibody (DakoCytomation, P0447, lot 00058737)
    Full Protocol
    • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
    • Transfect cells with 0.05nmol mouse Creb1 Silencer Select siRNA (ThermoFisher Scientific, ASO1093M, ASO10982 and ASO109CG, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions.
    • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, containing protease inhibitors (Sigma)).
    • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
    • Denature 5µg of total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing, freshly cast 10% SDS-PAGE for 1h at 140V.
    • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
    • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
    • Incubation with primary mouse anti-CREB1 antibody (antibodies-online, ABIN969061, lot 110517) diluted 1:10000 in TBST containing 5% milk for 1h at RT.
    • Wash membrane 5x 5min with TBST.
    • Incubation with anti-mouse horseradish peroxidase-conjugated secondary antibody (DakoCytomation, P0447, lot 00058737) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
    • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
    • Reveal protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
    • Strip membranes using RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
    • Wash and reveal the membrane as described above.
    Notes
    The CREB1 antibody ABIN969061 reveals a protein of the expected molecular weight of murine Creb1 in lysates of primary mouse microglia. The protein band’s intensity decreases upon siRNA knockdown.
  • Format
    Liquid
    Buffer
    Ascitic fluid containing 0.03 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    4°C, -20°C for long term storage
  • Jansson, Ng, Fu, Depatie, Al Azzabi, Screaton: "Glucose controls CREB activity in islet cells via regulated phosphorylation of TORC2." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 29, pp. 10161-6, (2008) (PubMed).

    Mamdani, Alda, Grof, Young, Rouleau, Turecki: "Lithium response and genetic variation in the CREB family of genes." dans: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 147B, Issue 4, pp. 500-4, (2008) (PubMed).

    Seo, Chung: "CREB activates proteasomal degradation of DSCR1/RCAN1." dans: FEBS letters, Vol. 582, Issue 13, pp. 1889-93, (2008) (PubMed).

  • Antigène
    CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
    Autre désignation
    CREB1 (CREB1 Produits)
    Synonymes
    anticorps creb, anticorps CREB1, anticorps Creb1, anticorps CREB, anticorps 2310001E10Rik, anticorps 3526402H21Rik, anticorps AV083133, anticorps Creb, anticorps Creb-1, anticorps creb1, anticorps zgc:55598, anticorps cAMP responsive element binding protein 1, anticorps cAMP responsive element binding protein, anticorps cAMP responsive element binding protein 1 S homeolog, anticorps cAMP responsive element binding protein 1a, anticorps CREB1, anticorps creb1, anticorps CREB, anticorps Creb1, anticorps creb1.S, anticorps creb1a
    Sujet

    Description: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)

    Aliases: CREB, MGC9284, CREB1

    Poids moléculaire
    43 kDa
    ID gène
    1385
    HGNC
    1385
    Pathways
    Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
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