anti-Souris DFFA anticorps pour Enzyme Immunoassay

Recommended DFFA Antibody (fourni par: Connectez-vous pour afficher )

Antigène
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Anticorps
  • DFF-45
  • DFF1
  • ICAD
  • A330085O09Rik
  • DFF35
  • Dff45
  • ICAD-L
  • ICAD-S
  • DFFA
  • zgc:92229
  • DNA fragmentation factor subunit alpha
  • DNA fragmentation factor, alpha subunit
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • DNA fragmentation factor, alpha polypeptide
  • DFFA
  • Dffa
  • dffa
Reactivité
Humain, Souris, Rat (Rattus)
169
84
42
2
2
1
1
1
Hôte
Lapin
194
33
2
Clonalité
Polyclonal
Conjugué
Cet anticorp DFFA est non-conjugé
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
197
74
62
36
34
31
17
13
6
5
4
3
2
2
1
Fournisseur
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N° du produit (Fournisseur)
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N° du produit ABIN272297
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
1 ABIN5554855 EIA IHC (p) WB Rabbit IgG N-Term Connectez-vous pour afficher Polyclonal 0
1 ABIN5554856 EIA WB Rabbit IgG C-Term Connectez-vous pour afficher Polyclonal 0

General

Antigène DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Anticorps
Reactivité Humain, Souris, Rat (Rattus)
(169), (84), (42), (2), (2), (1), (1), (1)
Hôte Lapin
(194), (33), (2)
Clonalité
Polyclonal
Conjugué Cet anticorp DFFA est non-conjugé
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(197), (74), (62), (36), (34), (31), (17), (13), (6), (5), (4), (3), (2), (2), (1)
Fournisseur Connectez-vous pour afficher

Détail du produit anti-DFFA anticorps

Détail du antigène DFFA Information d'application Stockage Images
Specificité This antibody detects endogenous levels of ICAD protein. (region surrounding Gln177)
Réactivité croisée (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Pureté > 95 % pure by SDS-PAGE
Plasmids, Primers & others

Détail du antigène DFFA

Détail du produit anti-DFFA anticorps Information d'application Stockage Images Haut de la page
Antigène
Autre désignation DFFA / ICAD (DFFA Antibody Extrait)
Sujet The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a 112 kDa nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature 85 kDa apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the 45 kDa subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The 70 kDa nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD
Poids moléculaire approx. 36 kDa
ID gène 13347
NCBI Accession NP_034174
UniProt O54786
Pathways Apoptose, Caspase Cascade in Apoptosis

Information d'application

Détail du produit anti-DFFA anticorps Détail du antigène DFFA Stockage Images Haut de la page
Indications d'application ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Stockage

Détail du produit anti-DFFA anticorps Détail du antigène DFFA Information d'application Images Haut de la page
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation DO NOT FREEZE!
Stock 4 °C
Stockage commentaire Store the antibody undiluted at 2-8 °C.

Images

Détail du produit anti-DFFA anticorps Détail du antigène DFFA Information d'application Stockage Haut de la page
Images (Fournisseur)
 image for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody (ABIN272297) anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody
 image for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody (ABIN272297) anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody (Image 2)