Recevoir ce produit gratuitement
Envoyez-nous votre proposition de validation. J'aimerais valider ce produit
General |
|
---|---|
Antigène | Poly (ADP-Ribose) Polymerase 1 (PARP1) Anticorps |
Reactivité | Toutes les espèces Alternatives |
Hôte | Souris Alternatives |
Clonalité (Clone) | |
Conjugué | Cet anticorp PARP1 est non-conjugé Alternatives |
Application |
Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
Alternatives
|
Fournisseur | Connectez-vous pour afficher |
Détail du produit anti-PARP1 anticorpsDétail du antigène PARP1 Information d'application Stockage Images |
|
Purification | Chromatography |
Clone | 10H |
Isotype | IgG3 kappa |
Plasmids, Primers & others | |
Détail du antigène PARP1Détail du produit anti-PARP1 anticorps Information d'application Stockage Images Haut de la page |
|
Antigène | |
Autre désignation | PARP (PARP1 Antibody Extrait) |
Sujet |
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP's N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro. Synonyms: PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1 |
ID gène | 142 |
NCBI Accession | NP_001609 |
Pathways | Apoptose, Caspase Cascade in Apoptosis, Réparation de l'ADN, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location |
Information d'applicationDétail du produit anti-PARP1 anticorps Détail du antigène PARP1 Stockage Images Haut de la page |
|
Indications d'application |
Immunocytochemistry : 5-20 μg/mL Western Blot (10 μg/mL using colorimetric methods <2 μg/mL for ECL) Immunoprecipitation and ELISA |
Commentaires |
Suggested positive control: |
Restrictions | For Research Use only |
StockageDétail du produit anti-PARP1 anticorps Détail du antigène PARP1 Information d'application Images Haut de la page |
|
Format | Liquid |
Concentration | 1.0 mg/mL |
Buffer | 1 mg/mL of IgG purified from mouse ascites by protein A chromatography. Prepared in 20 mM Tris, pH 7.4, 150 mM NaCl, 1 % BSA and 0.02 % sodium azide. |
Agent conservateur | Sodium azide |
Précaution d'utilisation | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Conseil sur la manipulation | The antibody solution should be gently mixed before use. |
Stock | -20 °C |
Stockage commentaire | For long-term storage, aliquot and freeze at -70 °C. |
Date de péremption | 12 months |
ImagesDétail du produit anti-PARP1 anticorps Détail du antigène PARP1 Information d'application Stockage Haut de la page |
|
Images (Fournisseur) |