anti-Rat (Rattus) AIRE anticorps pour Immunocytochemistry

Recommended AIRE Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Autoimmune Regulator (AIRE) Anticorps
  • AIRE1
  • APECED
  • APS1
  • APSI
  • PGA1
  • autoimmune regulator
  • autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy)
  • AaeL_AAEL013794
  • CpipJ_CPIJ001208
  • CpipJ_CPIJ001209
  • AIRE
  • Aire
Reactivité
Humain, Souris, Rat (Rattus)
167
73
29
11
9
8
7
4
4
2
1
Hôte
Lapin
113
61
10
1
1
Clonalité
Polyclonal
Conjugué
Cet anticorp AIRE est non-conjugé
7
6
6
6
5
5
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
163
103
50
39
17
14
12
11
5
5
4
2
1
1
1
Fournisseur
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N° du produit (Fournisseur)
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N° du produit ABIN6266320
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
1 ABIN2615296 ICC IF WB Rabbit Internal Region Connectez-vous pour afficher Polyclonal 0

General

Antigène Autoimmune Regulator (AIRE) Anticorps
Reactivité Humain, Souris, Rat (Rattus)
(167), (73), (29), (11), (9), (8), (7), (4), (4), (2), (1)
Hôte Lapin
(113), (61), (10), (1), (1)
Clonalité
Polyclonal
Conjugué Cet anticorp AIRE est non-conjugé
(7), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(163), (103), (50), (39), (17), (14), (12), (11), (5), (5), (4), (2), (1), (1), (1)
Fournisseur Connectez-vous pour afficher

Détail du produit anti-AIRE anticorps

Détail du antigène AIRE Information d'application Stockage Images
Specificité AIRE Antibody detects endogenous levels of total AIRE
 Réactivité croisée Humain, Souris, Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogène A synthetic peptideof human AIRE
Isotype IgG
Plasmids, Primers & others

Détail du antigène AIRE

Détail du produit anti-AIRE anticorps Information d'application Stockage Images Haut de la page
Antigène
Autre désignation AIRE (AIRE Antibody Extrait)
Sujet

Description: Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA) (PubMed:26084028). Binds to G-doublets in an A/T-rich environment, the preferred motif is a tandem repeat of 5'-ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (By similarity). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla (PubMed:26084028). Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program. Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression. Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes. In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection. In secondary lymphoid organs, induces functional inactivation of CD4+ T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli (By similarity).

Gene: AIRE

Poids moléculaire 58kDa
ID gène 326
UniProt O43918
Pathways Chromatin Binding

Information d'application

Détail du produit anti-AIRE anticorps Détail du antigène AIRE Stockage Images Haut de la page
Indications d'application WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Stockage

Détail du produit anti-AIRE anticorps Détail du antigène AIRE Information d'application Images Haut de la page
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20 °C.Stable for 12 months from date of receipt
Date de péremption 12 months

Images

Détail du produit anti-AIRE anticorps Détail du antigène AIRE Information d'application Stockage Haut de la page
Images (Fournisseur)
Western Blotting (WB) image for anti-Autoimmune Regulator (AIRE) antibody (ABIN6266320) Western blot analysis of Hela whole cell lysates, using AIRE Antibody. The lane on th...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Autoimmune Regulator (AIRE) antibody (ABIN6266320) ABIN6276866 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeab...
Immunohistochemistry (IHC) image for anti-Autoimmune Regulator (AIRE) antibody (ABIN6266320) ABIN6276866 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehy...